Stability of [−2]Pro‐PSA in Whole Blood and Serum: Analysis for Optimal Measurement Conditions. Issue 4 (27th February 2014)

• Record Type: Journal Article
• Authors: Igawa, Tsukasa; Takehara, Kosuke; Onita, Toru; Ito, Kazuto; Sakai, Hideki
• Is Part Of: Journal of clinical laboratory analysis. Volume 28:Issue 4(2014:Jul.)
• Page Start: 315
• Page End: 319
• Publication Date: 2014-02-27
• Subjects: Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.21687
• Languages: English
• ISSNs: 0887-8013

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jcla21687-sec-0010" sec-type="section"> <title>Background</title> <p>The clinical usefulness of [−2]pro‐PSA (where PSA is prostate‐specific antigen) in prostate cancer diagnosis has been emphasized in recent studies. To determine proper blood sample handling conditions for [−2]pro‐PSA evaluation, we analyzed the preanalytical stability of [−2]pro‐PSA.</p> </sec> <sec id="jcla21687-sec-0020" sec-type="section"> <title>Methods</title> <p>Blood samples from 22 Japanese males were stored under various conditions before total PSA (tPSA), free PSA, and [−2]pro‐PSA concentrations were measured, and the preanalytical stability of [−2]pro‐PSA and the changes in the Prostate Health Index (<italic>phi</italic>) were assessed.</p> </sec> <sec id="jcla21687-sec-0030" sec-type="section"> <title>Results</title> <p>[−2]Pro‐PSA was stable in serum for at least 24 hr at both room temperature (RT) and at 4°C. However, [−2]pro‐PSA levels in whole blood increased rapidly over time, particularly at RT. Mean recovery (%) of [−2]pro‐PSA in whole blood at RT was >110% at 1 hr after drawing of blood. The <italic>phi</italic> tended to increase over time in a pattern similar to the change in[−2]pro‐PSA.</p> </sec> <sec id="jcla21687-sec-0040" sec-type="section"> <title>Conclusions</title> <p>Preanalytical stability was lower for [−2]pro‐PSA than for free PSA or tPSA. Whole‐blood [−2]pro‐PSA increased in a time‐dependent manner, particularly at RT. Thus, whole blood samples collected at RT should be centrifuged within 1 hr after drawing. The [−2]pro‐PSA in serum is stable for at least 24 hr at both RT and at 4°C.</p> </sec> </abstract>