MRD detection of leukemia relapse using HLA typing by FACS in combination with FISH after mismatched allogeneic stem cell transplantation. (12th May 2014)

• Record Type: Journal Article
• Authors: Miyachi, Mitsuru; Watanabe, Eri; Watanabe, Nobukazu; Tsuma, Yusuke; Kawashima‐Goto, Sachiko; Tamura, Shinichi; Imamura, Toshihiko; Ishida, Hiroyuki; Hosoi, Hajime
• Is Part Of: Pediatric transplantation. Volume 18:Number 5(2014:Aug.)
• Page Start: E180
• Page End: E184
• Publication Date: 2014-05-12
• Subjects: Transplantation of organs, tissues, etc. in children -- Periodicals; 617.95408305
• DOI: 10.1111/petr.12267
• Languages: English
• ISSNs: 1397-3142

Abstract

<abstract abstract-type="main" id="petr12267-abs-0001"> <title>Abstract</title> <p>Loss of mismatched HLA is a cause of relapse following HLA‐mismatched allo‐SCT. We directly detected the loss of mismatched HLA alleles in relapsed leukemic cells at a MRD level using HLA typing by multicolor FACS (HLA‐Flow) in combination with FISH in the BM of two patients with <italic>MLL‐AF9‐</italic>positive AML, at 6 and 10 months after mismatched allo‐SCT. HLA‐Flow with FISH analysis detected relapsed leukemic cells not expressing a mismatched HLA allele and harboring the <italic>MLL</italic> rearrangement. Simultaneously, real‐time quantitative RT‐PCR detected a low copy number of <italic>MLL‐AF9</italic> transcripts, consistent with MRD detection. HLA‐Flow with FISH is a powerful method for detecting molecular relapse after mismatched allo‐SCT and provides important information on the HLA expression status of the relapsed leukemic cells to help determine the next intervention.</p> </abstract>