Pharmacokinetics and ex‐vivo pharmacodynamics of cefquinome against Klebsiella pneumonia in healthy dogs. Issue 4 (24th December 2013)

• Record Type: Journal Article
• Authors: Zhang, B.; Gu, X.; Li, X.; Gu, M.; Zhang, N.; Shen, X.; Li, Y.; Ding, H.
• Is Part Of: Journal of veterinary pharmacology and therapeutics. Volume 37:Issue 4(2014)
• Page Start: 367
• Page End: 373
• Publication Date: 2013-12-24
• Subjects: Veterinary pharmacology -- Periodicals; Therapeutics -- Periodicals; 636.0895105
• DOI: 10.1111/jvp.12100
• Languages: English
• ISSNs: 0140-7783

Abstract

<abstract abstract-type="main" id="jvp12100-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A two‐period cross‐over study was carried to investigate the pharmacokinetics (PK) and <italic>ex‐vivo</italic> pharmacodynamics (PD) of cefquinome when administrated intravenously (IV) and intramuscularly (IM) in seven healthy dogs at a dose of 2 mg/kg of body weight. Serum concentrations were determined by HPLC‐MS/MS assay and cefquinome concentration vs. time data after IV and IM were best fit to a two‐compartment open model. Cefquinome mean values of area under concentration–time curve (AUC) were 5.15 <italic>μ</italic>g·h/mL for IV dose and 4.59 <italic>μ</italic>g·h/mL for IM dose. Distribution half‐lives and elimination half‐lives after IV dose and IM dose were 0.27 and 0.44 h, 1.53 and 1.94 h, respectively. Values of total body clearance (Cl<sub>B</sub>) and volume of distribution at steady‐state (<italic>V</italic><sub>ss</sub>) were 0.49 L·kg/h and 0.81 L/kg, respectively. After IM dose, <italic>C</italic><sub>max</sub> was 2.53 <italic>μ</italic>g/mL and the bioavailability was 89.13%. For PD profile, the determined MIC and MBC values against <italic>K. pneumonia</italic> were 0.030 and 0.060 <italic>μ</italic>g/mL in MHB and 0.032 and 0.064 <italic>μ</italic>g/mL in serum. The <italic>ex vivo</italic> time‐kill curves also were established in serum. In conjunction with the data on MIC, MBC values and the <italic>ex vivo</italic> bactericidal activity in serum, the present results allowed prediction that a single cefquinome dosage of 2 mg/kg may be effective in dogs against <italic>K. pneumonia</italic> infection.</p> </abstract>