Immunomodulatory factors and infectious agents associated with the hepatic gene expression of the IGF system in nursery pigs. (27th February 2014)

• Record Type: Journal Article
• Authors: Slifierz, M. J.; Friendship, R.; de Lange, C. F. M.; Slavic, D.; Grgic, H.; Farzan, A.
• Is Part Of: Animal. Volume 8:Number 5(2014:May)
• Page Start: 844
• Page End: 851
• Publication Date: 2014-02-27
• Subjects: Animal breeding -- Periodicals; Animal genetics -- Periodicals; Animal nutrition -- Periodicals; Animal physiology -- Periodicals; Environmental sciences -- Periodicals; 636.005
• DOI: 10.1017/S1751731114000275
• Languages: English
• ISSNs: 1751-7311

Abstract

<abstract abstract-type="normal"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <p>Recent findings suggest there is a complex interaction between the IGF system and the inflammatory immune response. The objective of this study was to determine whether gene expression of growth factors (IGF-1, IGF-binding protein-3 (IGFBP-3) and growth hormone receptors (GHR)) in the liver is associated with gene expression of immunomodulators in the liver, including C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), interferon-<italic>α</italic> (IFN-<italic>α</italic>), IFN-<italic>γ</italic>, tumour necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin (IL)-1<italic>β</italic>, IL-6, IL-10 and IL-18, as well as with the presence of <italic>Salmonella</italic> spp., <italic>Lawsonia intracellularis</italic>, <italic>Brachyspira</italic> spp., enterotoxigenic <italic>Escherichia coli</italic>, methicillin-resistant <italic>Staphylococcus aureus</italic>, swine influenza virus, and porcine reproductive and respiratory syndrome virus (PRRSV) in nursery pigs (<italic>n</italic>=74) from commercial farms (<italic>n</italic>=4). Gene expression was quantified using reverse transcription quantitative-PCR (RT-qPCR) and the data were modelled using logistic regression methods. Pigs with elevated IGF-1 expression were less likely to have increased expression of TNF-<italic>α</italic> (odds ratio (OR)=0.14, <italic>P</italic><0.01) and IL-18 (OR=0.19, <italic>P</italic><0.05), and less likely to be colonized with PRRSV (OR=0.03, <italic>P</italic><0.01). Pigs with increased expression of IGFBP-3 were more likely to have elevated IL-6 expression (OR=8.5, <italic>P</italic><0.05). It was also observed that IGFBP-3 and IGF-1 were significantly associated when Hp expression was low (OR=30, <italic>P</italic><0.05), but this association was not significant when Hp expression was high (<italic>P</italic>=0.54). Pigs with increased expression of GHR were less likely to have elevated expression of SAA (OR=0.01, <italic>P</italic><0.05) and IL-1β (OR=0.03, <italic>P</italic><0.05), but more likely to have increased expression of CRP (OR=290, <italic>P</italic><0.01). Overall, there appears to be an inverse association between the hepatic expression of the IGF system (IGF-1, IGFBP-3, GHR) and certain cytokines (IL-1<italic>β</italic>, IL-18, TNF-<italic>α</italic>) and acute-phase proteins (SAA, Hp).</p> </abstract>