Towards tender X‐rays with Zernike phase‐contrast imaging of biological samples at 50 nm resolution. (11th June 2014)

• Record Type: Journal Article
• Authors: Vartiainen, Ismo; Warmer, Martin; Goeries, Dennis; Herker, Eva; Reimer, Rudolph; David, Christian; Meents, Alke
• Is Part Of: Journal of synchrotron radiation. Volume 21:Part 4(2014)
• Page Start: 790
• Page End: 794
• Publication Date: 2014-06-11
• Subjects: Synchrotron radiation -- Periodicals; Free electron lasers -- Periodicals; 539.73505
• DOI: 10.1107/S1600577514010388
• Languages: English
• ISSNs: 0909-0495

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>X‐ray microscopy is a commonly used method especially in material science application, where the large penetration depth of X‐rays is necessary for three‐dimensional structural studies of thick specimens with high‐<italic>Z</italic> elements. In this paper it is shown that full‐field X‐ray microscopy at 6.2 keV can be utilized for imaging of biological specimens with high resolution. A full‐field Zernike phase‐contrast microscope based on diffractive optics is used to study lipid droplet formation in hepatoma cells. It is shown that the contrast of the images is comparable with that of electron microscopy, and even better contrast at tender X‐ray energies between 2.5 keV and 4 keV is expected.</p> </abstract>