Sample Preparation for Liquid Chromatographic Analysis of Phytochemicals in Biological Fluids. Issue 4 (2nd December 2013)

• Record Type: Journal Article
• Authors: Oh, Ju‐Hee; Lee, Young‐Joo; Choi, Young Hae; Verpoorte, Robert
• Is Part Of: Phytochemical analysis. Volume 25:Issue 4(2014:Jul.)
• Page Start: 314
• Page End: 330
• Publication Date: 2013-12-02
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2484
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2484-sec-0001" sec-type="section"> <title>Introduction</title> <p>Natural products have been used traditionally for the treatment and prevention of diseases for thousands of years and are nowadays consumed as dietary supplements and herbal medicine. To ensure the safe and effective use of these herbal products, information about bioavailability of active compounds in plasma or target tissues should be provided via validated analytical methods combined with appropriate sampling methods.</p> </sec> <sec id="pca2484-sec-0002" sec-type="section"> <title>Objective</title> <p>To provide comprehensive and abridged information about sample preparation methods for the quantification of phytochemicals in biological samples using liquid chromatography analysis.</p> </sec> <sec id="pca2484-sec-0003" sec-type="section"> <title>Methods</title> <p>Sample pre‐treatment procedures used in analytical methods for <italic>in vivo</italic> pharmacokinetic studies of natural compounds or herbal medicines were reviewed. These were categorised according to the biological matrices (plasma, bile, urine, faeces and tissues) and sample clean‐up processes (protein precipitation, liquid–liquid extraction and solid‐phase extraction).</p> </sec> <sec id="pca2484-sec-0004" sec-type="section"> <title>Results</title> <p>Although various kinds of sample pre‐treatment methods have been developed, liquid–liquid extraction is still widely used and solid‐phase extraction is becoming increasingly popular because of its efficiency for extensive clean up of complex matrix samples. However, protein precipitation is still favoured due to its simplicity.</p> </sec> <sec id="pca2484-sec-0005" sec-type="section"> <title>Conclusion</title> <p>Sample treatment for phytochemical analysis in biological fluids is an indispensable and critical step to obtain high quality results. This step could dominate the overall analytical process because both the duration of the process as well as the reliability of the data depend in large part on its efficiency. Thus, special attention should be given to the choice of a proper sample treatment method that targets analytes and their biomatrix. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>