Mapping brain circuit function in vivo using two‐photon fluorescence microscopy. Issue 7 (7th February 2014)

• Record Type: Journal Article
• Authors: Bovetti, Serena; Moretti, Claudio; Fellin, Tommaso; Cella Zanacchi, Francesca; Bianchini, Paolo; Vicidomini, Giuseppe
• Is Part Of: Microscopy research and technique. Volume 77:Issue 7(2014:Jul.)
• Page Start: 492
• Page End: 501
• Publication Date: 2014-02-07
• Subjects: Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22342
• Languages: English
• ISSNs: 1059-910X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Mapping the activity of neuronal circuits with high resolution in the intact brain is a fundamental step toward understanding brain function. In the last several years, nonlinear microscopy combined with fluorescent activity reporters has become a crucial tool for achieving this goal. In this review article, we will highlight the principles underlying nonlinear microscopy and discuss its application to neuroscience, focusing on recent functional studies in the rodent neocortex in combination with genetically encoded calcium indicators. <italic>Microsc. Res. Tech. 77:492–501, 2014</italic>. © 2014 Wiley Periodicals, Inc.</p> </abstract>