3‐Dimensional Tissue Is Formed From Cancer Cells In Vitro on Gelfoam®, But Not on MatrigelTM. Issue 8 (August 2014)

• Record Type: Journal Article
• Authors: Tome, Yasunori; Uehara, Fuminari; Mii, Sumiyuki; Yano, Shuya; Zhang, Lei; Sugimoto, Naotoshi; Maehara, Hiroki; Bouvet, Michael; Tsuchiya, Hiroyuki; Kanaya, Fuminori; Hoffman, Robert M.
• Is Part Of: Journal of cellular biochemistry. Volume 115:Issue 8(2014:Aug.)
• Page Start: 1362
• Page End: 1367
• Publication Date: 2014-08
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.24780
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb24780-sec-0001" sec-type="section"> <p>Cell and tissue culture can be performed on different substrates such as on plastic, in Matrigel™, and on Gelfoam<sup>®</sup>, a sponge matrix. Each of these substrates consists of a very different surface, ranging from hard and inflexible, a gel, and a sponge‐matrix, respectively. Folkman and Moscona found that cell shape was tightly coupled to DNA synthesis and cell growth. Therefore, the flexibility of a substrate is important for cells to maintain their optimal shape. Human osteosarcoma cells, stably expressing a fusion protein of α<sub>v</sub> integrin and green fluorescent protein (GFP), grew as a simple monolayer without any structure formation on the surface of a plastic dish. When the osteosarcoma cells were cultured within Matrigel™, the cancer cells formed colonies but no other structures. When the cancer cells were seeded on Gelfoam<sup>®</sup>, the cells formed three‐dimensional tissue‐like structures. The behavior of 143B osteosarcoma cells on Gelfoam<sup>®</sup> in culture is remarkably different from those of these cells in monolayer culture or in Matrigel™. Tissue‐like structures were observed only in Gelfoam<sup>®</sup> culture. The data in this report suggest a flexible structural substrate such as Gelfoam<sup>®</sup> provides a more in vivo‐like culture condition than monolayer culture or Matrigel<sup>TM</sup> and that Matrigel<sup>TM</sup> does not result in actual three‐dimensional culture. J. Cell. Biochem. 115: 1362–1367, 2014. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>