Distinct roles for hu li tai shao and swallow in cytoskeletal organization during Drosophila oogenesis. Issue 7 (16th April 2014)

• Record Type: Journal Article
• Authors: Pokrywka, Nancy Jo; Zhang, Huadi; Raley‐Susman, Kathleen
• Is Part Of: Developmental dynamics. Volume 243:Issue 7(2014:Jul.)
• Page Start: 906
• Page End: 916
• Publication Date: 2014-04-16
• Subjects: Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24132
• Languages: English
• ISSNs: 1058-8388

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <underline>Background:</underline> Cytoskeletal organization is essential for localization of developmentally significant molecules during <italic>Drosophila</italic> oogenesis. Swallow (Swa) and an isoform of Hu li tai shao (Ovhts‐RC) have been implicated in the organization of actin filaments in developing oocytes but their precise roles have been obscured by the dependence of <italic>hts</italic> RNA localization on <italic>swa</italic> function. The functional significance of <italic>hts</italic> RNA localization in the oocyte has not been established. <underline>Results:</underline> In this study we examine Ovhts‐RC distribution and cytoskeletal organization under conditions in which Swa protein and/or <italic>hts</italic> RNA localization are perturbed. We find Swa is required for overall actin organization and for the maintenance of a distinct subset of microtubules in the oocyte. <italic>hts</italic> RNA localization modulates the distribution of Ovhts‐RC in the oocyte and, in turn, local actin filament proliferation. <underline>Conclusions:</underline> Our results support separate contributions of Swa and <italic>hts</italic> RNA localization to actin organization during oogenesis. Swa is crucial for the organization of actin networks that lead to the formation of a specialized microtubule population, while Ovhts‐RC acts to modulate spatially restricted actin filament growth at the oocyte cortex. This suggests RNA localization can lead to modifications of both the actin and microtubule cytoskeletons at specific subcellular locales. <italic>Developmental Dynamics 243:906–916, 2014</italic>. © 2014 The Authors Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists</p> </abstract>