Spatially Selective Heteronuclear Multiple‐Quantum Coherence Spectroscopy for Biomolecular NMR Studies. Issue 9 (30th April 2014)

• Record Type: Journal Article
• Authors: Sathyamoorthy, Bharathwaj; Parish, David M.; Montelione, Gaetano T.; Xiao, Rong; Szyperski, Thomas
• Is Part Of: Chemphyschem. Volume 15:Issue 9(2014)
• Page Start: 1872
• Page End: 1879
• Publication Date: 2014-04-30
• Subjects: Chemistry, Physical and theoretical -- Periodicals; 541.05
• DOI: 10.1002/cphc.201301232
• Languages: English
• ISSNs: 1439-4235

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Spatially selective heteronuclear multiple‐quantum coherence (SS HMQC) NMR spectroscopy is developed for solution studies of proteins. Due to "time‐staggered" acquisitioning of free induction decays (FIDs) in different slices, SS HMQC allows one to use long delays for longitudinal nuclear spin relaxation at high repetition rates. To also achieve high intrinsic sensitivity, SS HMQC is implemented by combining a single spatially selective ¹H excitation pulse with nonselective ¹H 180° pulses. High‐quality spectra were obtained within 66 s for a 7.6 kDa uniformly ¹³C, ¹⁵N‐labeled protein, and within 45 and 90 s for, respectively, two proteins with molecular weights of 7.5 and 43 kDa, which were uniformly ²H, ¹³C, ¹⁵N‐labeled, except for having protonated methyl groups of isoleucine, leucine and valine residues.</p> </abstract>