Perinatal bisphenol A exposures increase production of pro-inflammatory mediators in bone marrow-derived mast cells of adult mice. (July 2014)

• Record Type: Journal Article
• Authors: O'Brien, Edmund; Dolinoy, Dana C.; Mancuso, Peter
• Is Part Of: Journal of immunotoxicology. Volume 11:Number 3(2014)
• Page Start: 205
• Page End: 212
• Publication Date: 2014-07
• Subjects: Immunotoxicology -- Periodicals; Poisons -- Immunology -- Periodicals; Environmental health -- Periodicals; 616.97
• DOI: 10.3109/1547691X.2013.822036
• Languages: English
• ISSNs: 1547-691X

Abstract

<abstract> <title>Abstract</title> <p>Bisphenol A (BPA) is a widely used monomer of polycarbonate plastics and epoxide resin that has been implicated in asthma pathogenesis when exposure occurs to the developing fetus. However, few studies have examined the relationship between perinatal BPA exposure and asthma pathogenesis in adulthood. This study used an isogenic mouse model to examine the influence of perinatal BPA exposure via maternal diet on inflammatory mediators associated with asthma in 6-month-old adult offspring by measuring bone marrow-derived mast cell (BMMC) production of lipid mediators (cysteinyl leukotrienes and prostaglandin D<sub>2</sub>), cytokines (interleukin [IL]-4, IL-5, IL-6, IL-13, and tumor necrosis factor [TNF]-<italic>α</italic>), and histamine. Global DNA methylation levels in BMMCs from adult offspring were determined to elucidate a potential regulatory mechanism linking perinatal exposure to mast cell phenotype later in life. Four BPA exposure doses were tested: low (50 ng BPA/kg diet, <italic>n</italic> = 5), medium (50 μg BPA/kg diet, <italic>n</italic> = 4), high (50 mg BPA/kg diet, <italic>n</italic> = 4), and control (<italic>n</italic> = 3). Following BMMC activation, increases in cysteinyl leukotriene (<italic>p</italic> < 0.01) and TNF<italic>α</italic> (<italic>p</italic> < 0.05) production were observed in all BPA-exposure groups, and increases in prostaglandin D<sub>2</sub> (<italic>p</italic> < 0.01) and IL-13 (<italic>p</italic> < 0.01) production were observed in the high exposure group. Additionally, BMMCs from adult mice in all exposure groups displayed a decrease in global DNA methylation compared to control animals. Thus, perinatal BPA exposure displayed a long-term influence on mast cell-mediated production of pro-inflammatory mediators associated with asthma and global DNA methylation levels, suggesting a potential for mast cell dysregulation, which could affect pulmonary inflammation associated with allergic airway disease into adulthood.</p> </abstract>