Electrochemical Monitoring of the Early Events of Hydrogen Peroxide Production by Mitochondria1. Issue 26 (22nd May 2014)

• Record Type: Journal Article
• Authors: Suraniti, Emmanuel; Ben‐Amor, Salem; Landry, Pauline; Rigoulet, Michel; Fontaine, Eric; Bottari, Serge; Devin, Anne; Sojic, Neso; Mano, Nicolas; Arbault, Stéphane
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 26(2014)
• Page Start: 6655
• Page End: 6658
• Publication Date: 2014-05-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201403096
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Mitochondria consume oxygen in the respiratory chain and convert redox energy into ATP. As a side process, they produce reactive oxygen species (ROS), whose physiological activities are still not understood. However, current analytical methods cannot be used to monitor mitochondrial ROS quantitatively and unambiguously. We have developed electrochemical biosensors based on peroxidase‐redox polymer‐modified electrodes, providing selective detection of H₂O₂ with nanomolar sensitivity, linear response over five concentration decades, and fast response time. The release of H₂O₂ by mitochondria was then monitored under phosphorylating or inhibited respiration conditions. We report the detection of two concomitant regimes of H₂O₂ release: large fluxes (hundreds of n<sc>M</sc>) under complex III inhibition, and bursts of a few n<sc>M</sc> immediately following mitochondria activation. These unprecedented bursts of H₂O₂ are assigned to the role of mitochondria as the hub of redox signaling in cells.</p> </abstract>