A Smart Library of Epoxide Hydrolase Variants and the Top Hits for Synthesis of (S)‐β‐Blocker Precursors1. Issue 26 (19th May 2014)

• Record Type: Journal Article
• Authors: Kong, Xu‐Dong; Ma, Qian; Zhou, Jiahai; Zeng, Bu‐Bing; Xu, Jian‐He
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 26(2014)
• Page Start: 6641
• Page End: 6644
• Publication Date: 2014-05-19
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201402653
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Microtuning of the enzyme active pocket has led to a smart library of epoxide hydrolase variants with an expanded substrate spectrum covering a series of typical β‐blocker precursors. Improved activities of 6‐ to 430‐fold were achieved by redesigning the active site at two predicted hot spots. This study represents a breakthrough in protein engineering of epoxide hydrolases and resulted in enhanced activity toward bulky substrates.</p> </abstract>