Different effects of P. gingivalis LPS and E. coli LPS on the expression of interleukin-6 in human gingival fibroblasts. (July 2013)

• Record Type: Journal Article
• Authors: Andrukhov, Oleh; Ertlschweiger, Sandra; Moritz, Andreas; Bantleon, Hans-Peter; Rausch-Fan, Xiaohui
• Is Part Of: Acta odontologica Scandinavica. Volume 72:Number 5(2014)
• Page Start: 337
• Page End: 345
• Publication Date: 2013-07
• Subjects: Dentistry -- Periodicals; 617.6005
• DOI: 10.3109/00016357.2013.834535
• Languages: English
• ISSNs: 0001-6357

Abstract

<abstract> <title>Abstract</title> <p> <bold> <italic>Objective.</italic> </bold> Gingival fibroblasts (GFs) produce pro-inflammatory cytokines in response to stimulation with lipopolysaccharide (LPS) of <italic>Porphyromonas gingivalis, </italic> which is thought to be mediated by activation of toll-like receptors (TLR)2 and TLR4. The present study investigated the expression of interleukin (IL)-6, TLR2, and TLR4 in GFs of seven different donors upon stimulation with <italic>P. gingivalis</italic> LPS. The effects of <italic>P. gingivalis</italic> LPS were compared with those of TLR4 agonist <italic>Escherichia coli</italic> LPS and TLR2 agonist Pam3CSK4. <bold><italic>Materials and methods.</italic></bold> GFs were stimulated with <italic>P. gingivalis</italic> LPS, <italic>E. coli</italic> LPS or Pam3CSK4 and the expression of IL-6, TLR2 and TLR4 was measured by qPCR. The surface expression of TLR2 and TLR4 was measured by flow cytometry. <italic><bold>Results</bold></italic>. In GFs from three donors, <italic>P. gingivalis</italic> LPS and Pam3CSK4 induced a markedly lower increase in IL-6 expression than <italic>E. coli</italic> LPS. This was accompanied by significant down-regulation of the TLR2 and TLR4 expression. In GFs from another four donors, an increase in IL-6 expression upon stimulation with <italic>P. gingivalis</italic> LPS and Pam3CSK4 was similar or even higher than that induced by <italic>E. coli</italic> LPS. In GFs of these donors, all stimuli induced an up-regulation of both mRNA and protein expression of TLR2 and did not influence that of TLR4. <bold><italic>Conclusions.</italic></bold> This study suggests that <italic>P. gingivalis</italic> LPS and <italic>E. coli</italic> LPS differently regulate cytokine production in human gingival fibroblasts. Regulation of the expression level of TLR2 and TLR4 by periodontal pathogens might be an important factor controlling the inflammatory response in GFs.</p> </abstract>