Isoprostane and isofuran lipid mediators accumulate in stored red blood cells and influence platelet function in vitro. Issue 6 (5th November 2013)

• Record Type: Journal Article
• Authors: Spinelli, Sherry L.; Lannan, Katie L.; Casey, Ann E.; Croasdell, Amanda; Curran, Timothy M.; Henrichs, Kelly F.; Pollock, Stephen J.; Milne, Ginger A.; Refaai, Majed A.; Francis, Charles W.; Phipps, Richard P.; Blumberg, Neil
• Is Part Of: Transfusion. Volume 54:Issue 6(2014)
• Page Start: 1569
• Page End: 1579
• Publication Date: 2013-11-05
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.12485
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="trf12485-sec-0001" sec-type="section"> <title>Background</title> <p>Stored red blood cells (RBCs) release hemoglobin (Hb) that leads to oxidative damage, which may contribute to thrombosis in susceptible transfusion recipients. Oxidative stress stimulates the generation of a new class of lipid mediators called F<sub>2</sub>‐isoprostanes (F<sub>2</sub>‐IsoPs) and isofurans (IsoFs) that influence cellular behavior. This study investigated RBC‐derived F<sub>2</sub>‐IsoPs and IsoFs during storage and their influence on human platelets (PLTs).</p> </sec> <sec id="trf12485-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>F<sub>2</sub>‐IsoP and IsoF levels in RBC supernatants were measured by mass spectrometry during storage and after washing. The effects of stored supernatants, cell‐free Hb, or a key F<sub>2</sub>‐IsoP, 8‐iso‐prostaglandin F<sub>2α</sub> (PGF<sub>2α</sub>), on PLT function were examined in vitro.</p> </sec> <sec id="trf12485-sec-0003" sec-type="section"> <title>Results</title> <p>F<sub>2</sub>‐IsoPs, IsoFs, and Hb accumulated in stored RBC supernatants. Prestorage leukoreduction reduced supernatant F<sub>2</sub>‐IsoPs and IsoFs levels, which increased again over storage time. Stored RBC supernatants and 8‐iso‐PGF<sub>2α</sub> induced PLT activation marker CD62P (P‐selectin) expression and prothrombotic thromboxane A<sub>2</sub> release. Cell‐free Hb did not alter PLT mediator release, but did inhibit PLT spreading. Poststorage RBC washing reduced F<sub>2</sub>‐IsoP and IsoF levels up to 24 hours.</p> </sec> <sec id="trf12485-sec-0004" sec-type="section"> <title>Conclusions</title> <p>F<sub>2</sub>‐IsoPs and IsoFs are produced by stored RBCs and induce adverse effects on PLT function in vitro, supporting a potential novel role for bioactive lipids in adverse transfusion outcomes. F<sub>2</sub>‐IsoP and IsoF levels could be useful biomarkers for determining the suitability of blood components for transfusion. A novel finding is that cell‐free Hb inhibits PLT spreading and could adversely influence wound healing. Poststorage RBC washing minimizes harmful lipid mediators, and its use could potentially reduce transfusion complications.</p> </sec> </abstract>