Lack of transglutaminase 2 diminished T‐cell responses in mice. Issue 3 (July 2014)

• Record Type: Journal Article
• Authors: Kim, Jin‐Hee; Hong, Jun Man; Jeong, Eui Man; Lee, Wang Jae; Kim, Hang‐Rae; Kang, Jae Seung; Kim, In‐Gyu; Hwang, Young‐il
• Is Part Of: Immunology. Volume 142:Issue 3(2014:Jul.)
• Page Start: 506
• Page End: 516
• Publication Date: 2014-07
• Subjects: Immunology -- Periodicals
• DOI: 10.1111/imm.12282
• Languages: English
• ISSNs: 0019-2805

Abstract

<abstract abstract-type="main" id="imm12282-abs-0001"> <title>Summary</title> <p>Transglutaminase 2 (TG2) has been reported to play a role in dendritic cell activation and B‐cell differentiation after immunization. Its presence and role in T cells, however, has not been explored. In the present study, we determined the expression of TG2 on mouse T cells, and evaluated its role by comparing the behaviours of wild‐type and TG2<sup>−/−</sup> T cells after activation. In our results, naive T cells minimally expressed TG2, expression of which was increased after activation. T‐cell proliferation, expression of activation markers such as CD69 and CD25, and secretions of interleukin‐2 and interferon‐<italic>γ</italic> were suppressed in the absence of TG2, presumably due, in part, to diminished nuclear factor‐<italic>κ</italic>B activation. These effects on T cells seemed to be reflected in the <italic>in vivo</italic> immune response, the contact hypersensitivity reaction elicited by 2, 4‐dinitro‐1‐fluorobenzene, with lowered peak responses in the TG2<sup>−/−</sup> mice. When splenic T cells from mice immunized with tumour lysate‐loaded wild‐type dendritic cells were re‐challenged <italic>ex vivo</italic> with the same antigen, the profile of surface markers including CD44, CD62L, and CD127 strongly indicated lesser generation of memory CD8<sup>+</sup> T cells in TG2<sup>−/−</sup> mice. In the TG2<sup>−/−</sup> CD8<sup>+</sup> T cells, moreover, Eomes expression was markedly decreased. These results indicate possible roles of TG2 in CD8<sup>+</sup> T‐cell activation and CD8<sup>+</sup> memory T‐cell generation.</p> </abstract>