High-density lipoprotein subfractions and influence of endothelial lipase in a healthy Turkish population: A study in a land of low high-density lipoprotein cholesterol. (June 2014)

• Record Type: Journal Article
• Authors: Kilic, Harun; Atalar, Enver; Lay, Incilay; Yazihan, Nuray; Buyukcam, Fatih; Saygisunar, Ugur; Aksoy, Murat; Gunduz, Huseyin; Akdemir, Ramazan
• Is Part Of: Scandinavian journal of clinical & laboratory investigation. Volume 74:Number 4(2014)
• Page Start: 278
• Page End: 284
• Publication Date: 2014-06
• Subjects: Clinical biochemistry -- Periodicals; Physiology, Pathological -- Periodicals; Physiology, Experimental -- Periodicals; Medicine -- Research -- Periodicals; Clinical medicine -- Periodicals; 616.0072
• DOI: 10.3109/00365513.2013.879992
• Languages: English
• ISSNs: 0036-5513

Abstract

<abstract> <title>Abstract</title> <p> <bold> <italic>Purpose</italic>.</bold> Low concentration of high-density lipoprotein (HDL) is prevalent in Turkey. Endothelial lipase (EL) regulates lipoprotein metabolism. Small, lipid-poor HDL particles represent more-efficient cholesterol acceptors than their large, lipid-rich counterparts. The aim of this study was to investigate HDL subfractions and the effect of EL on HDL concentrations in healthy Turkish population. <bold><italic>Methods</italic>.</bold> 102 healthy subjects were included in the study (mean age 33.6 ± 10.3 years, 42 female). HDL subfractions were assayed by single precipitation method and EL concentrations were measured by competitive enzyme immunoassay. <bold><italic>Results</italic>.</bold> Mean HDL concentrations were 1.45 ± 0.37 mmol/L in women, 1.10 ± 0.30 mmol/L in men. Small HDL subfraction levels did not differ statistically between < 1 mmol/L and ≥ 1.6 mmol/L total HDL groups. Small HDL was not correlated with EL, low density lipoprotein cholesterol (LDL), triglyceride (TG) and age but positively correlated with total cholesterol and HDL (<italic>r</italic> = 0.2, <italic>p</italic> = 0.017; <italic>r</italic> = 0.2, <italic>p</italic> = 0.028, respectively). Large HDL was not correlated with age, EL and total cholesterol, and negatively correlated with HDL, LDL, TG (<italic>r</italic> = − 0.7, <italic>p</italic> < 0.001; <italic>r</italic> = − 0.2, <italic>p</italic> = 0.045; <italic>r</italic> = − 0.3, <italic>p</italic> < 0.001, respectively). If subjects were divided into two groups as HDL< 1 mmol/L and HDL > 1.6 mmol/L, mean EL concentrations were 475.83 ± 521.77 nmol/L and 529.71 ± 276.92 nmol/L, respectively (<italic>p</italic> = 0.086). <bold><italic>Conclusion</italic>.</bold> There were no differences between small HDL concentrations in the HDL low and high groups. Our data did not support EL to be the reason for low HDL in a healthy Turkish population. Our results in a healthy population may serve as a reference for clinical studies on HDL subfractions.</p> </abstract>