Tmem27 is upregulated by vitamin D in INS-1 cells and its serum concentrations are low in patients with autoimmune diabetes. (June 2014)

• Record Type: Journal Article
• Authors: Pepaj, Milaim; Gjerlaugsen, Nina; Julien, Kari; Thorsby, Per M.
• Is Part Of: Scandinavian journal of clinical & laboratory investigation. Volume 74:Number 4(2014)
• Page Start: 358
• Page End: 365
• Publication Date: 2014-06
• Subjects: Clinical biochemistry -- Periodicals; Physiology, Pathological -- Periodicals; Physiology, Experimental -- Periodicals; Medicine -- Research -- Periodicals; Clinical medicine -- Periodicals; 616.0072
• DOI: 10.3109/00365513.2014.898322
• Languages: English
• ISSNs: 0036-5513

Abstract

<abstract> <title>Abstract</title> <p>Transmembrane protein 27 (Tmem27), which is expressed in pancreatic β-cells, plays an important role in insulin secretion and pancreatic β-cell proliferation. Analysis of the INS-1 cell proteome using stable isotope labeling by amino acids in cell culture (SILAC) in combination with LC-MS identified Tmem27 as the one of most robustly (up to seven-fold) upregulated proteins after treatment with the active metabolite of vitamin D, 1, 25-(OH)₂D₃. Furthermore, we report that Tmem27 which is cleaved and released from, i.e. pancreatic β-cells, is present in human serum and its levels are significantly lower in subjects with autoimmune diabetes as compared to healthy individuals (13% of the levels). Additionally, Tmem27 correlated positively (0.70) with C-peptide serum levels in healthy subjects. Our data indicate that Tmem27 could be of potential value as a serum marker for the pathogenesis of diabetes and as such may warrant the development of measurement methods with lower limit of detection for its further validation.</p> </abstract>