Dynamic MRI of rat brain following manganese administration through the internal carotid artery. (July 2014)

• Record Type: Journal Article
• Authors: Zhang, Run; Fa, Zhiqiang; Liu, Yi; Sun, Haitao; Li, Peng; Li, Shaorui; Wang, Xuxia; Lei, Hao; Jiang, Xiaodan
• Is Part Of: Neurological research. Volume 36:Number 7(2014)
• Page Start: 679
• Page End: 686
• Publication Date: 2014-07
• Subjects: Neurology -- Periodicals; Neurosciences -- Periodicals; 616.8005
• DOI: 10.1179/1743132813Y.0000000291
• Languages: English
• ISSNs: 0161-6412

Abstract

<abstract> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <sec> <title>Objective:</title> <p>In this study, we investigated the dynamic distribution processes of Mn<sup>2+</sup> in rat brain using magnetic resonance imaging (MRI) after an intra-arterial (IA) injection of MnCl<sub>2</sub> and following the breaking of the blood–brain barrier (BBB).</p> </sec> <sec> <title>Methods:</title> <p>Adult male Sprague-Dawley (SD) rats were employed in the study. After the rats were anesthetized with urethane, 25% mannitol was administrated into the right internal carotid artery (ICA) in order to disrupt the BBB, 50 mmol/l (10 mg/kg) of MnCl<sub>2</sub> was then injected into ICA prior to the MRI assay. The MRI was performed at 7 T for a continuous 2 hours following the administration of MnCl<sub>2</sub>. Image reconstruction and analysis were performed using the Statistical Parametric Mapping (SPM) software.</p> </sec> <sec> <title>Results:</title> <p>As time progressed, the Mn<sup>2+</sup> enhanced signal intensity showed a gradual increase with a maximum increase at 10 minutes following MnCl<sub>2</sub> administration, and began to decline after 10 minutes. From 30 minutes to 2 hours, the signal-enhanced region became more homogeneous, and the signal-enhanced range spread to the contralateral area up to 2 hours after MnCl<sub>2</sub> administration.</p> </sec> <sec> <title>Conclusion:</title> <p>These results will help future research to select an appropriate time point to perform functional MRI for different types of activity-induced manganese (AIM) MRI research studies. These findings will allow researchers to discriminate intended, stimulation-specific enhanced signal from unintended, nonspecific enhanced signals.</p> </sec> </abstract>