Analysis of SPATA19 gene expression during male germ cells development, lessons from in vivo and in vitro study. (12th December 2013)

• Record Type: Journal Article
• Authors: Nourashrafeddin, Seyedmehdi; Ebrahimzadeh‐Vesal, Reza; Miryounesi, Mohammad; Aarabi, Mahmoud; Zarghami, Nosratollah; Modarressi, Mohammad Hosein; Nouri, Mohammad
• Is Part Of: Cell biology international reports. Volume 21:Number 1(2014)
• Page Start: 1
• Page End: 7
• Publication Date: 2013-12-12
• Subjects: Cytology -- Periodicals; 571.605
• DOI: 10.1002/cbi3.10010
• Languages: English
• ISSNs: 2041-5346

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="cbi310010-sec-0001" sec-type="section"> <p>Spermatogenesis‐associated‐19 (<italic>SPATA19</italic>) is a novel spermatogenesis related gene that has important biological functions in reproduction. However, the expression aspects of <italic>SPATA19</italic> gene are not yet well understood. We analysed <italic>SPATA19</italic> gene expression during mouse testis development in vivo (5, 15 and 25 days old mouse testis), in parallel with mouse embryonic stem cells (ESCs) differentiation into male germ‐like cells in vitro (5, 11, 19 and 27 days after differentiation), as well as Sertoli cell, mouse embryonic fibroblasts (MEF), and NIH3T3 cancerous cell line. We cultured transgenic mouse ESCs line C57BL/6J expressing Stra8‐EGFP in ESC's medium and induced their differentiation by retinoic acid (RA) treatment, and meiotic cells were then sorted by FACS. RT‐PCR and real‐time PCR were used for analysis of <italic>SPATA19</italic> mRNA expression. The expression of <italic>SPATA19</italic> mRNA is increased during mouse testis development in vivo and it is highest in 25‐days‐old mouse testis. <italic>SPATA19</italic> is also expressed most highly in mouse ESC‐derived germ‐like cells after 27 days of RA induction and NIH3T3 cell line but no expression was found in mouse ESC and Sertoli cell line. These findings demonstrate that the expression of <italic>SPATA19</italic> increases during male germ cells development. We also suggest an additional possible role for <italic>SPATA19</italic> in male germ cell differentiation and tumourigenic process.</p> </sec> </abstract>