Hypoxia induces osteogenesis in rabbit adipose‐derived stem cells overexpressing bone morphogenic protein‐2. (19th July 2013)

• Record Type: Journal Article
• Authors: Xu, L; Sun, X; Cao, K; Wu, Y; Zou, D; Liu, Y; Zhang, X; Zhang, X; Wang, G; Huang, Q; Jiang, X
• Is Part Of: Oral diseases. Volume 20:Number 5(2014:Jul.)
• Page Start: 430
• Page End: 439
• Publication Date: 2013-07-19
• Subjects: Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.12148
• Languages: English
• ISSNs: 1354-523X

Abstract

<abstract abstract-type="main" id="odi12148-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="odi12148-sec-0001" sec-type="section"> <title>Objective</title> <p>Hypoxic culture potentiates mesenchymal stem cells (MSCs) to survive and secrete various growth factors. Genetically modified stem cells overexpressing bone morphogenic protein‐2 (BMP‐2) demonstrate strong osteogenic ability. Hence, we investigated the coeffect of hypoxic culture conditions and BMP‐2 overexpression on the osteogenic ability of rabbit adipose‐derived stem cells (rASCs) <italic>in vitro</italic>.</p> </sec> <sec id="odi12148-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Rabbit adipose‐derived stem cells with or without adenoviral‐BMP‐2 transduction were cultured in hypoxic (1%) and normoxic (21%) conditions. Cell viability, attachment, and proliferation were compared. Real‐time PCR amplification of osteogenic and angiogenic genes including alkaline phosphatase (ALP), osteocalcin (OCN), HIF‐1α, and vascular endothelial growth factor (VEGF) was performed. Moreover, ALP activity, immunofluorescent staining of OCN, and mineralization assay by alizarin red S quantification and von Kossa staining were conducted.</p> </sec> <sec id="odi12148-sec-0003" sec-type="section"> <title>Results</title> <p>Cells under hypoxic conditions attached better within 12 h and proliferated faster. While BMP‐2 overexpression and hypoxic condition separately elevated the transcription of key osteogenic and angiogenic genes, a cooperative effect was observed to enhance the upregulation of osteogenic as well as angiogenic genes. Identical changes were observed in ALP activity, immunofluorescent staining of OCN, and mineralization assay.</p> </sec> <sec id="odi12148-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Hypoxic culture can enhance the osteogenic ability of BMP‐2 gene‐modified rASCs, which provides a strategy to improve the osteogenesis of rASCs for <italic>in vivo</italic> bone regeneration.</p> </sec> </abstract>