Effects of dietary lipid levels on mitochondrial gene expression in low and high‐feed efficient families of rainbow trout Oncorhynchus mykiss. Issue 6 (June 2014)

• Record Type: Journal Article
• Authors: Eya, J. C.; Yossa, R.; Ashame, M. F.; Pomeroy, C. F.; Gannam, A. L.
• Is Part Of: Journal of fish biology. Volume 84:Issue 6(2014)
• Page Start: 1708
• Page End: 1720
• Publication Date: 2014-06
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12391
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12391-abs-0001"> <title>Abstract</title> <p id="jfb12391-para-0001">A 2 × 3 factorial study was conducted to evaluate the effects of dietary lipid level on mitochondrial gene expression in mixed sex rainbow trout <italic>Oncorhynchus mykiss</italic>. Practical diets with a fixed crude protein content of 42%, formulated to contain 10% (42/10), 20% (42/20) and 30% (42/30) dietary lipid, were fed to triplicate groups of either low‐feed efficient (F129; mean ± <sc>s.d.</sc> = 105·67 ± 3·04 g initial average mass) or high‐feed efficient (F134; mean ± <sc>s.d.</sc> = 97·86 ± 4·02 g) families of fish, to apparent satiety, twice per day, for 108 days. At the end of the experiment, diets 42/20 and 42/30 led to similar fish condition factors, which were higher than that observed with diet 42/10 (<italic>P</italic> < 0·05). F134 fish fed diet 42/10 showed the highest hepato‐somatic index, while there was no significant difference among all the other treatments (<italic>P</italic> < 0·05). When the group of F134 fish fed diet 42/10 was used as the calibrator for gene expression analysis, the five genes selected for their involvement in lipid metabolism (complex I‐<italic>nd1</italic>, complex III‐<italic>cytb</italic>, complex IV‐<italic>cox1</italic>, complex IV‐<italic>cox2</italic> and complex V‐<italic>atp6</italic>) were up‐regulated in the muscle and down‐regulated in both the liver and the intestine. There was a significant family × diet interaction regarding <italic>nd1</italic>, <italic>cox2</italic> and <italic>atp6</italic> in the liver; <italic>nd1</italic>, <italic>cytb</italic>, <italic>cox1</italic>, <italic>cox2</italic> and <italic>atp6</italic> in the intestine, and <italic>nd1</italic>, <italic>cytb</italic>, <italic>cox1</italic>, <italic>cox2</italic> and <italic>atp6</italic> in the muscle (<italic>P</italic> < 0·05). The overall results of this study constitute basic information for the understanding of molecular mechanisms of lipid metabolism at the mitochondrial level in fishes.</p> </abstract>