Effects of oral intake of hydrogen water on liver fibrogenesis in mice. Issue 6 (18th June 2013)

• Record Type: Journal Article
• Authors: Koyama, Yukinori; Taura, Kojiro; Hatano, Etsuro; Tanabe, Kazutaka; Yamamoto, Gen; Nakamura, Kojiro; Yamanaka, Kenya; Kitamura, Koji; Narita, Masato; Nagata, Hiromitsu; Yanagida, Atsuko; Iida, Taku; Iwaisako, Keiko; Fujinawa, Hikohito; Uemoto, Shinji
• Is Part Of: Hepatology research. Volume 44:Issue 6(2014:Jun.)
• Page Start: 663
• Page End: 677
• Publication Date: 2013-06-18
• Subjects: Liver -- Diseases -- Periodicals; Liver Diseases -- Periodicals; Foie -- Maladies -- Périodiques; 616.362
• DOI: 10.1111/hepr.12165
• Languages: English
• ISSNs: 1386-6346

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="hepr12165-sec-0001" sec-type="section"> <title>Aim</title> <p>Liver fibrosis is the universal consequence of chronic liver diseases. Sustained hepatocyte injury initiates an inflammatory response, thereby activating hepatic stellate cells, the principal fibrogenic cells in the liver. Reactive oxygen species are involved in liver injury and are a promising target for treating liver fibrosis. Hydrogen water is reported to have potential as a therapeutic tool for reactive oxygen species‐associated disorders. This study aimed to investigate the effects of hydrogen water on liver fibrogenesis and the mechanisms underlying these effects.</p> </sec> <sec id="hepr12165-sec-0002" sec-type="section"> <title>Methods</title> <p>C57BL/6 mice were fed with hydrogen water or control water, and subjected to carbon tetrachloride, thioacetamide and bile duct ligation treatments to induce liver fibrosis. Hepatocytes and hepatic stellate cells were isolated from mice and cultured with or without hydrogen to test the effects of hydrogen on reactive oxygen species‐induced hepatocyte injuries or hepatic stellate cell activation.</p> </sec> <sec id="hepr12165-sec-0003" sec-type="section"> <title>Results</title> <p>Oral intake of hydrogen water significantly suppressed liver fibrogenesis in the carbon tetrachloride and thioacetamide models, but these effects were not seen in the bile duct ligation model. Treatment of isolated hepatocyte with 1 μg/mL antimycin A generated hydroxyl radicals. Culturing in the hydrogen‐rich medium selectively suppressed the generation of hydroxyl radicals in hepatocytes and significantly suppressed hepatocyte death induced by antimycin A; however, it did not suppress hepatic stellate cell activation.</p> </sec> <sec id="hepr12165-sec-0004" sec-type="section"> <title>Conclusion</title> <p>We conclude that hydrogen water protects hepatocytes from injury by scavenging hydroxyl radicals and thereby suppresses liver fibrogenesis in mice.</p> </sec> </abstract>