Assessment of adulteration of soybean proteins in dairy products by 2D microchip‐CE device. Issue 11 (24th February 2014)

• Record Type: Journal Article
• Authors: Wu, Ruige; Wang, Zhiping; Fung, Ying Sing; Peng Seah, Daphne Yen; Yeung, William Shu‐Biu; Cifuentes, Alejandro
• Is Part Of: Electrophoresis. Volume 35:Issue 11(2014:Jun.)
• Page Start: 1728
• Page End: 1734
• Publication Date: 2014-02-24
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201300559
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>To determine the adulteration of soybean proteins in dairy product, a microchip‐CE device was developed to isolate selected fraction of soybean and milk proteins in p<italic>I</italic> range from 5.5∼7.0 by 1D IEF, followed by ITP/CZE in the embedded capillary for preconcentration, separation and UV detection at 280 nm. Compared to IEF‐CZE without ITP preconcentration, the enhancement factor (EF) in detection of soybean proteins was 20 times. Adulteration of 0.1% soybean protein in total dairy proteins can be detected in less than 10 min.</p> </abstract>