Autocrine IL‐10 promotes human B‐cell differentiation into IgM‐ or IgG‐secreting plasmablasts. Issue 6 (11th March 2014)

• Record Type: Journal Article
• Authors: Heine, Guido; Drozdenko, Gennadiy; Grün, Joachim R.; Chang, Hyun‐Dong; Radbruch, Andreas; Worm, Margitta
• Is Part Of: European journal of immunology. Volume 44:Issue 6(2014:Jun.)
• Page Start: 1615
• Page End: 1621
• Publication Date: 2014-03-11
• Subjects: Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201343822
• Languages: English
• ISSNs: 0014-2980

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>B‐cell‐derived interleukin‐10 (IL‐10) is known to act in a paracrine fashion to suppress inflammation. Here, we show that IL‐10 also acts in an autocrine manner to regulate the differentiation of activated human B cells. We report that IL‐10 expression is not restricted to a dedicated B‐cell subset, but is induced transiently in peripheral human naïve, memory, and CD5⁺ B cells alike upon activation. Global transcriptome comparison of activated human B cells, secreting IL‐10 or not, identified 138 differentially regulated genes, most of which were associated with differentiation into antibody‐secreting cells and reflecting autocrine IL‐10 signaling. We monitored the differentiation of IL‐10‐secreting B cells and determined the effect of IL‐10‐blocking antibodies against its autocrine and paracrine signaling. IL‐10 signaling promoted the differentiation of activated IL‐10‐secreting B cells into IgM‐ or IgG‐secreting cells, but was dispensable for IgA secretion. Our data imply that B‐cell‐derived IL‐10 not only suppresses immune reactions via paracrine mechanisms, but can also contribute to the differentiation of IL‐10‐secreting B cells into IgM‐ and IgG‐secreting plasmablasts through both autocrine and paracrine signaling.</p> </abstract>