A suite of software for processing MicroED data of extremely small protein crystals. (1st June 2014)

• Record Type: Journal Article
• Authors: Iadanza, Matthew G.; Gonen, Tamir
• Is Part Of: Journal of applied crystallography. Volume 47:Part 3(2014:Jun.)
• Page Start: 1140
• Page End: 1145
• Publication Date: 2014-06-01
• Subjects: Crystallography -- Periodicals; 548.05
• DOI: 10.1107/S1600576714008073
• Languages: English
• ISSNs: 0021-8898

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Electron diffraction of extremely small three‐dimensional crystals (MicroED) allows for structure determination from crystals orders of magnitude smaller than those used for X‐ray crystallography. MicroED patterns, which are collected in a transmission electron microscope, were initially not amenable to indexing and intensity extraction by standard software, which necessitated the development of a suite of programs for data processing. The MicroED suite was developed to accomplish the tasks of unit‐cell determination, indexing, background subtraction, intensity measurement and merging, resulting in data that can be carried forward to molecular replacement and structure determination. This <italic>ad hoc</italic> solution has been modified for more general use to provide a means for processing MicroED data until the technique can be fully implemented into existing crystallographic software packages. The suite is written in Python and the source code is available under a GNU General Public License.</p> </abstract>