Live‐Cell Stimulated Raman Scattering Imaging of Alkyne‐Tagged Biomolecules1. Issue 23 (17th April 2014)

• Record Type: Journal Article
• Authors: Hong, Senlian; Chen, Tao; Zhu, Yuntao; Li, Ang; Huang, Yanyi; Chen, Xing
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 23(2014)
• Page Start: 5827
• Page End: 5831
• Publication Date: 2014-04-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201400328
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Alkynes can be metabolically incorporated into biomolecules including nucleic acids, proteins, lipids, and glycans. In addition to the clickable chemical reactivity, alkynes possess a unique Raman scattering within the Raman‐silent region of a cell. Coupling this spectroscopic signature with Raman microscopy yields a new imaging modality beyond fluorescence and label‐free microscopies. The bioorthogonal Raman imaging of various biomolecules tagged with an alkyne by a state‐of‐the‐art Raman imaging technique, stimulated Raman scattering (SRS) microscopy, is reported. This imaging method affords non‐invasiveness, high sensitivity, and molecular specificity and therefore should find broad applications in live‐cell imaging.</p> </abstract>