The role of insulin against hydrogen peroxide-induced oxidative damages in differentiated SH-SY5Y cells. (June 2014)
- Record Type:
- Journal Article
- Title:
- The role of insulin against hydrogen peroxide-induced oxidative damages in differentiated SH-SY5Y cells. (June 2014)
- Main Title:
- The role of insulin against hydrogen peroxide-induced oxidative damages in differentiated SH-SY5Y cells
- Authors:
- Ramalingam, Mahesh
Kim, Sung-Jin - Abstract:
- <abstract> <title>Abstract</title> <p>Exogenous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can easily penetrate into biological membranes and enhance the formation of other reactive oxygen species (ROS). In the present study, we have investigated the neuroprotective effects of insulin on H<sub>2</sub>O<sub>2</sub>-induced toxicity of retinoic acid (RA)-differentiated SH-SY5Y cells. To measure the changes in the cell viability of SH-SY5Y cells at different concentrations of H<sub>2</sub>O<sub>2</sub> for 24 h, a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT)-based assay was used and a 100 µM H<sub>2</sub>O<sub>2</sub> was selected to establish a model of H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Further assays showed that 24 h of 100 µM H<sub>2</sub>O<sub>2</sub>-induced significant changes in the levels of lactate dehydrogenase (LDH), nitric oxide (NO), ROS, and calcium ion (Ca<sup>2+</sup>) in neuronal cells, but insulin can effectively diminish the H<sub>2</sub>O<sub>2</sub>-induced oxidative damages to these cells. Moreover, cells treated with insulin increased H<sub>2</sub>O<sub>2</sub>-induced suppression of glutathione levels and exerted an apparent suppressive effect on oxidative products. The results of insulin treatment with SH-SY5Y cells increased the Bcl-2 levels and decreased the Akt levels. The treatment of insulin had played a protective effect on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress related to the Akt/Bcl-2<abstract> <title>Abstract</title> <p>Exogenous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) can easily penetrate into biological membranes and enhance the formation of other reactive oxygen species (ROS). In the present study, we have investigated the neuroprotective effects of insulin on H<sub>2</sub>O<sub>2</sub>-induced toxicity of retinoic acid (RA)-differentiated SH-SY5Y cells. To measure the changes in the cell viability of SH-SY5Y cells at different concentrations of H<sub>2</sub>O<sub>2</sub> for 24 h, a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT)-based assay was used and a 100 µM H<sub>2</sub>O<sub>2</sub> was selected to establish a model of H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Further assays showed that 24 h of 100 µM H<sub>2</sub>O<sub>2</sub>-induced significant changes in the levels of lactate dehydrogenase (LDH), nitric oxide (NO), ROS, and calcium ion (Ca<sup>2+</sup>) in neuronal cells, but insulin can effectively diminish the H<sub>2</sub>O<sub>2</sub>-induced oxidative damages to these cells. Moreover, cells treated with insulin increased H<sub>2</sub>O<sub>2</sub>-induced suppression of glutathione levels and exerted an apparent suppressive effect on oxidative products. The results of insulin treatment with SH-SY5Y cells increased the Bcl-2 levels and decreased the Akt levels. The treatment of insulin had played a protective effect on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress related to the Akt/Bcl-2 pathways.</p> </abstract> … (more)
- Is Part Of:
- Journal of receptor and signal transduction research. Volume 34:Number 3(2014)
- Journal:
- Journal of receptor and signal transduction research
- Issue:
- Volume 34:Number 3(2014)
- Issue Display:
- Volume 34, Issue 3 (2014)
- Year:
- 2014
- Volume:
- 34
- Issue:
- 3
- Issue Sort Value:
- 2014-0034-0003-0000
- Page Start:
- 212
- Page End:
- 220
- Publication Date:
- 2014-06
- Subjects:
- Cell receptors -- Periodicals
Cellular signal transduction -- Periodicals
571.6 - Journal URLs:
- http://informahealthcare.com/journal/rst ↗
http://informahealthcare.com ↗ - DOI:
- 10.3109/10799893.2013.876043 ↗
- Languages:
- English
- ISSNs:
- 1079-9893
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 5047.849000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 3349.xml