Spironolactone inhibits production of proinflammatory mediators in response to lipopolysaccharide via inactivation of nuclear factor-κB. (June 2014)

• Record Type: Journal Article
• Authors: Kato, Yoshiro; Kamiya, Hideki; Koide, Naoki; Odkhuu, Erdenezaya; Komatsu, Takayuki; Dagvadorj, Jargalsaikhan; Watarai, Atsuko; Kondo, Masaki; Kato, Koichi; Nakamura, Jiro; Yokochi, Takashi
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 36:Number 3(2014:Jun.)
• Page Start: 237
• Page End: 241
• Publication Date: 2014-06
• Subjects: Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.3109/08923973.2014.921690
• Languages: English
• ISSNs: 0892-3973

Abstract

<abstract> <title>Abstract</title> <p>The effect of spironolactone (SPIR) on lipopolysaccharide (LPS)-induced production of proinflammatory mediators was examined using RAW 264.7 macrophage-like cells and mouse peritoneal macrophages. SPIR significantly inhibited LPS-induced production of nitric oxide (NO), tumor necrosis factor-α and prostaglandin E2. The inhibition was not mediated by cell death. SPIR reduced the expression of an inducible NO synthase mRNA in response to LPS. SPIR significantly inhibited phosphorylation of p65 nuclear factor (NF)-κB in response to LPS. Furthermore, SPIR inhibited phosphorylation of IκB kinase (IKK) as an upstream molecule of NF-κB in response to LPS. LPS did not induce the production of aldosterone in RAW 264.7 cells. Taken together, SPIR is suggested to inhibit LPS-induced proinflammatory mediators <italic>via</italic> inactivation of IKK/NF-κB in LPS signaling.</p> </abstract>