Analysis and pharmacokinetic study of curdione in Rhizoma Curcumae by UPLC/QTOF/MS. (June 2014)

• Record Type: Journal Article
• Authors: Lv, Diya; Cao, Yan; Dong, Xin; Chen, Xiaofei; Lou, Ziyang; Chai, Yifeng
• Is Part Of: Biomedical chromatography. Volume 28:Number 6(2014:Jun.)
• Page Start: 782
• Page End: 787
• Publication Date: 2014-06
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3118
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>An ultra‐performance liquid chromatography–quadrupole time‐of‐flight/mass spectrometry (UPLC/QTOF/MS) method was established to determine the chemical components of <italic>curcuma rhizomes</italic> (Ezhu) and their pharmacokinetics. Chromatographic separation was performed by UPLC using a 1.8 µm column in order to obtain good resolution and increase the sensitivity of analysis. Accurate mass measurement within 5 ppm error for each ion produced in positive mode electrospray ionization and the subsequent QTOF product ions enabled 12 compounds to be identified. Several of the identified components, including <italic>β</italic>‐elemene, curcumol, germacrone and curdione, are thought to be the biologically active ingredients. Quantitative pharmacokinetic analysis was also carried out by UPLC/QTOF/MS. Using 20(<italic>S</italic>)‐protopanoxadiol as an internal standard, samples were prepared by protein precipitation with methanol. Chromatographic separation was performed on an Agilent Extend‐C<sub>18</sub> column (2.1 × 50 mm, 1.8 µm) with acetonitrile (0.1% formic acid)–water (0.1% formic acid) for gradient elution. Curdione calibration plots were linear over the range of 0.1–12.2 µg/mL for curdione in plasma with the lower quantification limit being 6.5 ng/mL, and the recovery from plasma was about 105.2%. The RSD for both intra‐ and inter‐day precision was <9.9%. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>