Isolation of a new broad spectrum antifungal polyene from Streptomyces sp. MTCC 5680. (11th March 2014)

• Record Type: Journal Article
• Authors: Vartak, A.; Mutalik, V.; Parab, R.R.; Shanbhag, P.; Bhave, S.; Mishra, P.D.; Mahajan, G.B.
• Is Part Of: Letters in applied microbiology. Volume 58:Number 6(2014:Jun.)
• Page Start: 591
• Page End: 596
• Publication Date: 2014-03-11
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12229
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12229-abs-0001"> <title>Abstract</title> <sec id="lam12229-sec-0101" sec-type="section"> <p>A new polyene macrolide antibiotic PN00053 was isolated from the fermentation broth of <italic>Streptomyces</italic> sp. wild‐type strain MTCC‐5680. The producer strain was isolated from fertile mountain soil of Naldehra region, Himachal Pradesh, India. The compound PN00053 was purified through various steps of chromatographic techniques and bio‐activity guided fractionation followed by its characterization using physiochemical properties, spectral data (<sup>1</sup>H‐NMR, <sup>13</sup>C‐NMR, HMBC, HSQC, and COSY) and MS analysis. PN00053 exhibited broad spectrum <italic>in vitro</italic> antifungal activity against strains of <italic>Aspergillus fumigatus</italic> (HMR), <italic>A. fumigatus </italic>ATCC 16424, <italic>Candida albicans</italic> (I.V.), <italic>C. albicans </italic>ATCC 14503, <italic>C. krusei </italic>GO6, <italic>C. glabrata </italic>HO4, <italic>Cryptococcus neoformans</italic>, <italic> Trichophyton</italic> sp. as well as fluconazole resistant strains <italic>C. krusei </italic>GO3 and <italic>C. glabrata </italic>HO5. It did not inhibit growth of gram positive and gram‐negative bacteria, displaying its specificity against fungi.</p> </sec> <sec id="lam12229-sec-0102" sec-type="section"> <title>Significance and Impact of the Study</title> <p>PN00053 is a novel polyene macrolide isolated from a wild strain of <italic>Streptomyces</italic> sp. PM0727240 (MTCC5680), an isolate from the mountainous rocky regions of Himachal Pradesh, India. The compound is a new derivative of the antibiotic Roflamycoin [32, 33–didehydroroflamycoin (DDHR)]. It displayed broad spectrum antifungal activity against yeast and filamentous fungi. However, it did not show any antibacterial activity. The <italic>in vitro</italic> study revealed that PN00053 has better potency as compared to clinical gold standard fluconazole. The development of pathogenic resistance against the polyenes has been seldom reported. Hence, we envisage PN00053 could be a potential antifungal lead.</p> </sec> </abstract>