Mosquitoes rely on their gut microbiota for development. Issue 11 (16th May 2014)

• Record Type: Journal Article
• Authors: Coon, Kerri L.; Vogel, Kevin J.; Brown, Mark R.; Strand, Michael R.
• Is Part Of: Molecular ecology. Volume 23:Issue 11(2014)
• Page Start: 2727
• Page End: 2739
• Publication Date: 2014-05-16
• Subjects: Molecular ecology -- Periodicals; Molecular population biology -- Periodicals; 576
• DOI: 10.1111/mec.12771
• Languages: English
• ISSNs: 0962-1083

Abstract

<abstract abstract-type="main" id="mec12771-abs-0001"> <title>Abstract</title> <p>Field studies indicate adult mosquitoes (Culicidae) host low diversity communities of bacteria that vary greatly among individuals and species. In contrast, it remains unclear how adult mosquitoes acquire their microbiome, what influences community structure, and whether the microbiome is important for survival. Here, we used pyrosequencing of 16S rRNA to characterize the bacterial communities of three mosquito species reared under identical conditions. Two of these species, <italic>Aedes aegypti</italic> and <italic>Anopheles gambiae</italic>, are anautogenous and must blood‐feed to produce eggs, while one, <italic>Georgecraigius atropalpus</italic>, is autogenous and produces eggs without blood feeding. Each mosquito species contained a low diversity community comprised primarily of aerobic bacteria acquired from the aquatic habitat in which larvae developed. Our results suggested that the communities in <italic>Ae. aegypti</italic> and <italic>An. gambiae</italic> larvae share more similarities with one another than with <italic>G. atropalpus</italic>. Studies with <italic>Ae. aegypti</italic> also strongly suggested that adults transstadially acquired several members of the larval bacterial community, but only four genera of bacteria present in blood fed females were detected on eggs. Functional assays showed that axenic larvae of each species failed to develop beyond the first instar. Experiments with <italic>Ae. aegypti</italic> indicated several members of the microbial community and <italic>Escherichia coli</italic> successfully colonized axenic larvae and rescued development. Overall, our results provide new insights about the acquisition and structure of bacterial communities in mosquitoes. They also indicate that three mosquito species spanning the breadth of the Culicidae depend on their gut microbiome for development.</p> </abstract>