Genome‐wide survey of transcriptional initiation in the pathogenic fungus, Candida glabrata. (14th April 2014)

• Record Type: Journal Article
• Authors: Aoyama, Toshihiro; Nakayama, Hironobu; Ueno, Keigo; Inukai, Tatsuya; Tanabe, Koichi; Nagi, Minoru; Bard, Martin; Chibana, Hiroji
• Is Part Of: Genes to cells. Volume 19:Number 6(2014:Jun.)
• Page Start: 478
• Page End: 503
• Publication Date: 2014-04-14
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12147
• Languages: English
• ISSNs: 1356-9597

Abstract

<abstract abstract-type="main" id="gtc12147-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>DNA sequencing of the 5′‐flanking region of the transcriptome effectively identifies transcription initiation sites and also aids in identifying unknown genes. This study describes a comprehensive polling of transcription start sites and an analysis of full‐length complementary DNAs derived from the genome of the pathogenic fungus <italic>Candida glabrata</italic>. A comparison of the sequence reads derived from a cDNA library prepared from cells grown under different culture conditions against the reference genomic sequence of the Candida Genome Database (CGD: <ext-link ext-link-type="uri" xlink:href="http://www.candidagenome.org/" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://www.candidagenome.org/</ext-link>) revealed the expression of 4316 genes and their acknowledged transcription start sites (TSSs). In addition this analysis also predicted 59 new genes including 22 that showed no homology to the genome of <italic>Saccharomyces cerevisiae</italic>, a genetically close relative of <italic>C. glabrata</italic>. Furthermore, comparison of the 5′‐untranslated regions (5′‐UTRs) and core promoters of <italic>C. glabrata</italic> to those of <italic>S. cerevisiae</italic> showed various global similarities and differences among orthologous genes. Thus, the <italic>C. glabrata</italic> transcriptome can complement the annotation of the genome database and should provide new insights into the organization, regulation, and function of genes of this important human pathogen.</p> </abstract>