A recombinant human hyaluronidase sustained release gel for the treatment of post‐surgical edema. (29th October 2013)

• Record Type: Journal Article
• Authors: Nekoroski, Tara; Paladini, Rudolph D.; Sauder, Daniel N.; Frost, Gregory I.; Keller, Gilbert‐A.
• Is Part Of: International journal of dermatology. Volume 53:Number 6(2014:Jun.)
• Page Start: 777
• Page End: 785
• Publication Date: 2013-10-29
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/ijd.12304
• Languages: English
• ISSNs: 0011-9059

Abstract

<abstract abstract-type="main" id="ijd12304-abs-0001"> <title>Abstract</title> <sec id="ijd12304-sec-0001" sec-type="section"> <title>Background</title> <p>Edema commonly accompanies surgical procedures and when excessive, can adversely affect surgical outcomes. The skin extracellular matrix, including one of its primary components, hyaluronan (HA), is a significant barrier to effective drainage of accumulated edematous fluid. Recombinant human hyaluronidase (rHuPH20) is a human hyaluronidase that acts transiently and locally to depolymerize HA. A non‐liposomal gel formulation that provides a sustained release of rHuPH20 was tested <italic>in vivo</italic> in a preclinical murine model of acquired lymphedema.</p> </sec> <sec id="ijd12304-sec-0002" sec-type="section"> <title>Methods</title> <p>Lymphedemic mice were injected 24 hours before surgery, and at 2 and 12 days following surgery with rHuPH20 sustained release gel (PH20 SR gel). Quantitative assessment of treatment response indicated that a single dose of PH20 SR gel resulted in accelerated resolution and reduced severity of post‐surgical edema as compared to the gel vehicle (control).</p> </sec> <sec id="ijd12304-sec-0003" sec-type="section"> <title>Results</title> <p>Statistically significant enzymatic degradation of HA was demonstrated up to 5 mm from the injection site, and histological analysis confirmed removal of HA up to 72 hours following PH20 SR gel administration.</p> </sec> <sec id="ijd12304-sec-0004" sec-type="section"> <title>Conclusions</title> <p>These results demonstrate sustained hyaluronidase enzymatic activity that promotes diffusion of accumulated post‐surgical edematous fluid, suggesting that PH20 SR gel may be a useful adjuvant in promoting postoperative edema resolution.</p> </sec> </abstract>