Rare‐Cell Enrichment by a Rapid, Label‐Free, Ultrasonic Isopycnic Technique for Medical Diagnostics1. Issue 22 (26th March 2014)

• Record Type: Journal Article
• Authors: Bourquin, Yannyk; Syed, Abeer; Reboud, Julien; Ranford‐Cartwright, Lisa C.; Barrett, Michael P.; Cooper, Jonathan M.
• Is Part Of: Angewandte Chemie international edition. Volume 53:Issue 22(2014)
• Page Start: 5587
• Page End: 5590
• Publication Date: 2014-03-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201310401
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>One significant challenge in medical diagnostics lies in the development of label‐free methods to separate different cells within complex biological samples. Here we demonstrate a generic, low‐power ultrasonic separation technique, able to enrich different cell types based upon their physical properties. For malaria, we differentiate between infected and non‐infected red blood cells in a fingerprick‐sized drop of blood. We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005 %). In a second example, we also show that our methods can be used to enrich different cell types, concentrating <italic>Trypanosoma</italic> in blood at very low levels of infection, on disposable, low‐cost chips.</p> </abstract>