Cloning, overexpression, purification and preliminary X‐ray analysis of a feast/famine regulatory protein (Rv2779c) from Mycobacterium tuberculosis H37Rv. Issue 1 (1st January 2014)

• Record Type: Journal Article
• Authors: Dey, Abhishek; Ramachandran, Ravishankar
• Is Part Of: Acta crystallographica. Volume 70:Issue 1(2014:Jan.)
• Page Start: 97
• Page End: 100
• Publication Date: 2014-01-01
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X13033128
• Languages: English
• ISSNs: 2053-230X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Rv2779c from <italic>Mycobacterium tuberculosis</italic> is a feast/famine regulatory protein. This class of proteins are also known as the leucine‐responsive regulatory protein/asparagine synthase C family (Lrp/AsnC) of transcriptional regulators and are known to be involved in various metabolic processes in bacteria and fungi. They contain a RAM (regulator of amino‐acid metabolism) domain that is rarely found in humans and acts as the oligomerization domain. Since the oligomeric status is often linked to the particular functional role in these proteins, binding of ligands to the domain can elicit specific functional responses. Full‐length Rv2779c corresponding to a molecular mass of 19.8 kDa and 179 residues was cloned and purified to homogeneity following transformation into <italic>Escherichia coli</italic> C41 (DE3) cells. Crystals were grown by vapour diffusion using the hanging‐drop method. Diffraction data extending to 2.8 Å resolution were collected from a single crystal that belonged to space group <italic>P</italic>2<sub>1</sub>2<sub>1</sub>2, with unit‐cell parameters <italic>a</italic> = 99.6, <italic>b</italic> = 146.0, <italic>c</italic> = 49.9 Å. Matthews coefficient (<italic>V</italic><sub>M</sub>) calculations suggest that four molecules are present in the asymmetric unit, corresponding to a solvent content of ∼46%. Molecular‐replacement calculations using the crystal structure of a homologue, Rv3291c, as the search model gave an unambiguous solution corresponding to four subunits in the asymmetric unit.</p> </abstract>