Validation of a novel real‐time PCR for detecting Rasamsonia argillacea species complex in respiratory secretions from cystic fibrosis patients. Issue 3 (20th April 2014)

• Record Type: Journal Article
• Authors: Steinmann, J.; Giraud, S.; Schmidt, D.; Sedlacek, L.; Hamprecht, A.; Houbraken, J.; Meis, J. F.; Bouchara, J. P.; Buer, J.; Rath, P.‐M.
• Is Part Of: New microbes and new infections. Volume 2:Issue 3(2014)
• Page Start: 72
• Page End: 78
• Publication Date: 2014-04-20
• Subjects: Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; Microbiology -- Periodicals; Communicable Diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1002/nmi2.44
• Languages: English
• ISSNs: 2052-2975

Abstract

<abstract abstract-type="main" id="nmi244-abs-0001"> <title>Abstract</title> <p>Members of the recently introduced fungal genus <italic>Rasamsonia</italic> (formerly included in the <italic>Geosmithia</italic> genus) have been described as emerging pathogens in immunosuppressed hosts or patients with cystic fibrosis (CF). <italic>Rasamsonia</italic> species have often been misidentified as <italic>Penicillium</italic> or <italic>Paecilomyces</italic> because of similar morphological characteristics. We validated a commercially available real‐time PCR assay (Primerdesign™, UK) for accurate detection of species from the <italic>Rasamsonia argillacea</italic> complex. First, we tested this assay with a collection of 74 reference strains and clinical isolates and then compared the PCR with cultures of 234 respiratory samples from 152 patients with CF from two University Hospitals in Germany and France. The assay reliably detected the three main species within the <italic>Rasamsonia argillacea</italic> species complex (<italic>R. argillacea</italic>, <italic> R. piperina</italic>, <italic> R. aegroticola</italic>), which are typically encountered in CF patients. The limit of DNA detection was between 0.01 and 1 pg/μL. Analysis of the DNA extracts from respiratory specimens of CF patients revealed that four out of the 153 patients studied (2.6%) were colonized with <italic>R. argillacea</italic> species complex. Two species from the <italic>R. argillacea</italic> complex grew in the parallel cultures from the same patients. In one patient the PCR was positive 5 months before culture. The real‐time PCR assay is a sensitive and specific method for detecting the three most important species of the <italic>R</italic>. <italic>argillacea</italic> species complex encountered in the CF context. Detection of these emerging pathogens in respiratory secretions from CF patients by this novel assay may increase our understanding of the occurrence and epidemiology of the <italic>R. argillacea</italic> species complex.</p> </abstract>