Analysis of TP53 Mutation Status in Human Cancer Cell Lines: A Reassessment. Issue 6 (6th May 2014)

• Record Type: Journal Article
• Authors: Leroy, Bernard; Girard, Luc; Hollestelle, Antoinette; Minna, John D.; Gazdar, Adi F.; Soussi, Thierry; Soussi, Thierry
• Is Part Of: Human mutation. Volume 35:Issue 6(2014:Jun.)
• Page Start: 756
• Page End: 765
• Publication Date: 2014-05-06
• Subjects: Human chromosome abnormalities -- Periodicals; Mutation (Biology) -- Periodicals; 616.04205
• DOI: 10.1002/humu.22556
• Languages: English
• ISSNs: 1059-7794

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="humu22556-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>We have used the 2014 release of the UMD TP53 database to show that TP53 status is still controversial for numerous cell lines, including some widely used lines from the NCI‐60 panel. Our analysis clearly confirms that, despite numerous warnings, the misidentification of cell lines is still present as a silent and neglected issue, and that extreme care must be taken when determining the status of p53, because errors may lead to disastrous experimental interpretations. A novel compendium gathering the TP53 status of 2, 500 cell lines has been made available (<ext-link ext-link-type="uri" xlink:href="http://p53.fr" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://p53.fr</ext-link>). <boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgh74wq6dh" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>