Disease‐specific and inflammation‐independent stromal alterations in spondylarthritis synovitis. Issue 1 (27th December 2012)

• Record Type: Journal Article
• Authors: Yeremenko, Nataliya; Noordenbos, Troy; Cantaert, Tineke; van Tok, Melissa; van de Sande, Marleen; Cañete, Juan D.; Tak, Paul P.; Baeten, Dominique
• Is Part Of: Arthritis and rheumatism. Volume 65:Issue 1(2013:Jan.)
• Page Start: 174
• Page End: 185
• Publication Date: 2012-12-27
• Subjects: Arthritis -- Periodicals; Rheumatism -- Periodicals; Arthritis -- Periodicals; Rheumatic Diseases -- Periodicals; Rhumatisme -- Périodiques; Arthrite -- Périodiques; 616.72
• DOI: 10.1002/art.37704
• Languages: English
• ISSNs: 0004-3591

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <sec id="abs1-1" sec-type="section"> <title>Objective</title> <p>The molecular processes driving the distinct patterns of synovial inflammation and tissue remodeling in spondylarthritis (SpA) as compared to rheumatoid arthritis (RA) remain largely unknown. Therefore, we aimed to identify novel and unsuspected disease‐specific pathways in SpA by a systematic and unbiased synovial gene expression analysis.</p> </sec> <sec id="abs1-2" sec-type="section"> <title>Methods</title> <p>Differentially expressed genes were identified by pan‐genomic microarray and confirmed by quantitative polymerase chain reaction and immunohistochemical analyses of synovial tissue biopsy samples from patients with SpA (n = 63), RA (n = 28), and gout (n = 9). The effect of inflammation on gene expression was assessed by stimulating fibroblast‐like synoviocytes (FLS) with synovial fluid and by analysis of synovial tissue samples at weeks 0 and 12 of etanercept treatment.</p> </sec> <sec id="abs1-3" sec-type="section"> <title>Results</title> <p>Using very stringent statistical thresholds, microarray analysis identified 64 up‐regulated transcripts in patients with SpA synovitis as compared to those with RA synovitis. Pathway analysis revealed a robust myogene signature in this gene set. The myogene signature was technically and biologically reproducible, was specific for SpA, and was independent of disease duration, treatment, and SpA subtype (nonpsoriatic versus psoriatic). Synovial tissue staining identified the myogene expressing cells as vimentin‐positive, prolyl 4‐hydroxylase β–positive, CD90+, and CD146+ mesenchymal cells that were significantly overrepresented in the intimal lining layer and synovial sublining of inflamed SpA synovium. Neither in vitro exposure to synovial fluid from inflamed SpA joints nor in vivo blockade of tumor necrosis factor modulated the SpA‐specific myogene signature.</p> </sec> <sec id="abs1-4" sec-type="section"> <title>Conclusion</title> <p>These data identify a novel and disease‐specific myogene signature in SpA synovitis. The fact that this stromal alteration appeared not to be downstream of local inflammation warrants further analysis of its functional role in the pathogenesis of the disease.</p> </sec> </abstract>