Bioactivity screening of five Centaurea species and in vivo anti-inflammatory activity of C. athoa. (June 2014)

• Record Type: Journal Article
• Authors: Erel, Sura Baykan; Demir, Serdar; Nalbantsoy, Ayse; Ballar, Petek; Khan, Shabana; Yavasoglu, N. Ulku Karabay; Karaalp, Canan
• Is Part Of: Pharmaceutical biology. Volume 52:Number 6(2014:Jun.)
• Page Start: 775
• Page End: 781
• Publication Date: 2014-06
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.868493
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Centaurea</italic> L. (Asteraceae) species used as herbal remedies in Turkish traditional medicine have shown several biological properties.</p> <p> <italic>Objective</italic>: Extracts obtained from the aerial parts of <italic>Centaurea aphrodisea</italic> Boiss., <italic>Centaurea athoa</italic> DC.<italic>, Centaurea hyalolepis</italic> Boiss<italic>., Centaurea iberica</italic> Trev<italic>.</italic> and <italic>Centaurea polyclada</italic> DC. were evaluated for their antioxidant, cytotoxic and anti-inflammatory activities.</p> <p> <italic>Materials and methods</italic>: Extracts of <italic>Centaurea</italic> species were tested for their antioxidant activity in the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2, 2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) screening assays and for i<italic>n vitro</italic> anti-inflammatory activity by Nf-κB and iNOS inhibition assays. The extracts were tested for their <italic>in vitro</italic> cytotoxicities against a panel of human solid tumor cell lines (SK-MEL: malignant melanoma, KB: oral epidermal carcinoma, BT-549: breast ductal carcinoma and SK-OV-3: ovary carcinoma) as well as non-cancerous kidney fibroblast (Vero) and kidney epithelial cells (LLC-PK1) by Neutral Red assay. <italic>In vivo</italic> anti-inflammatory activity of <italic>C. athoa</italic> was evaluated by the carrageenan-induced paw edema test in rats.</p> <p> <italic>Results</italic>: Antioxidant activities were observed for methanol extracts of plants. <italic>C. polyclada</italic> had the strongest effect on BT-549, KB and SK-OV-3 cell lines (30, 33 and 47 µg/ml, respectively). Nf-κB inhibition of chloroform extract of <italic>C. athoa</italic> was determined equivalent to positive control parthenolide (IC<sub>50</sub>: 6 µg/ml). This extract also showed anti-inflammatory activity by the carrageenan-induced paw edema test in rats, in all hours at a dose of 50 mg/kg compared to the control group.</p> <p> <italic>Discussion and conclusion</italic>: <italic>C. athoa</italic> is suggested to be a potential source of lead compounds for inflammatory diseases due to the significant <italic>in vitro</italic> and <italic>in vivo</italic> anti-inflammatory results.</p> </abstract>