A novel extraction of trichosanthin from Trichosanthes kirilowii roots using three-phase partitioning and its in vitro anticancer activity. (June 2014)

• Record Type: Journal Article
• Authors: Mondal, Arijit
• Is Part Of: Pharmaceutical biology. Volume 52:Number 6(2014:Jun.)
• Page Start: 677
• Page End: 680
• Publication Date: 2014-06
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.864684
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Three-phase partitioning (TPP), a unique technique which has been explored for protein separation, was used for extraction of trichosanthin (TCS).</p> <p> <italic>Objective</italic>: TPP was used to optimize the TCS extraction and to determine its anticancer activity.</p> <p> <italic>Materials and methods</italic>: The process consists of the simultaneous addition of <italic>t</italic>-butanol and ammonium sulfate to the aqueous slurry of <italic>Trichosanthes kirilowii</italic> Maxim (Cucurbitaceae) root powder. The extraction of TCS was optimized with respect to the concentration of ammonium sulfate loading, the ratio of <italic>t</italic>-butanol to slurry, extraction time and pH. The anticancer activity was performed using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay <italic>in vitro.</italic></p> <p> <italic>Results</italic>: The extraction time with this technique is lower in comparison to conventional solvent extraction. The optimized protocol resulted in maximum recovery of 98.68% (w/w) protein within 1 h. The <italic>in vitro</italic> cytotoxic activity of the TCS was evaluated against HepG2 and WRL 68 cancer cell line and results showed that TCS possesses quite highly significant anticancer activity having IC<sub>50</sub> values of 10.38 and 15.45 μmol/l, respectively, comparable to standard drugs.</p> <p> <italic>Conclusion</italic>: This framework is utilized as a basis for optimization for protein separation using TPP technique which is economical and eco-friendly.</p> </abstract>