Antinociceptive activity of Syzygium cumini leaves ethanol extract on orofacial nociception protocols in rodents. (June 2014)

• Record Type: Journal Article
• Authors: Quintans, Jullyana S. S.; Brito, Renan G.; Aquino, Pedro Gregório V.; França, Paulo H. B.; Siqueira-Lima, Pollyana S.; Santana, Antônio E. G.; Ribeiro, Eurica A. N.; Salvador, Marcos J.; Araújo-Júnior, João X.; Quintans-Júnior, Lucindo J.
• Is Part Of: Pharmaceutical biology. Volume 52:Number 6(2014:Jun.)
• Page Start: 762
• Page End: 766
• Publication Date: 2014-06
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.870582
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Syzygium cumini</italic> (L.) Skeels (Myrtaceae) is a tree with dark purple fruits, popularly known as "jambolão" or "jambolan". In folk medicine, this plant is used for the treatment of diabetes and inflammatory conditions.</p> <p> <italic>Objective</italic>: We investigated the antinociceptive effect of ethanol extract (EE) from <italic>S. cumini</italic> leaves on orofacial nociception.</p> <p> <italic>Material and methods</italic>: The antinociceptive effects of the EE obtained from the leaves of <italic>S. cumini</italic> were evaluated in mice using formalin- and glutamate-induced orofacial nociception.</p> <p> <italic>Results</italic>: ESI-MS/MS analyses demonstrated that major constituents in the analyzed samples coincided with the mass of the phenolic acids and flavonoids. In pharmacological approach, pre-treatment with EE (100, 200, or 400 mg/kg, p.o.) significantly reduced (<italic>p</italic> < 0.05 or <italic>p</italic> < 0.01) the percentage of paw licks time during phase 2 (43.2, 47.1, and 57.4%, respectively) of a formalin pain test when compared to control group animals. This effect was prevented by pretreatment with glibenclamide and N<sup>G</sup>-nitro-<sc>l</sc>-arginine (<sc>l</sc>-NOARG). The extract, all doses, also caused a marked inhibition (<italic>p</italic> < 0.01 or <italic>p</italic> < 0.001) of glutamate-induced orofacial nociception (38.8, 51.7, and 54.7%) when compared with the control group. No effect was observed with the rota-rod model.</p> <p> <italic>Conclusions</italic>: We can suggest that the antinociceptive effect of the EE is mediated by peripheral mechanisms, possibly involving K<sub>ATP</sub> channels and the nitric oxide pathways. These effects appear to be related to the presence of flavonoids compounds, such as quercetin.</p> </abstract>