Protective effects of raw and cooked blackcurrant extract on DNA damage induced by hydrogen peroxide in human lymphoblastoid cells. (June 2014)

• Record Type: Journal Article
• Authors: Yamamoto, Ayumi; Nakashima, Kana; Kawamorita, Saori; Sugiyama, Atena; Miura, Masanori; Kamitai, Yoshihiro; Kato, Yoji
• Is Part Of: Pharmaceutical biology. Volume 52:Number 6(2014:Jun.)
• Page Start: 782
• Page End: 788
• Publication Date: 2014-06
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.836721
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Blackcurrant (<italic>Ribes nigrum</italic> L.) is a classical fruit that has long been used to make juice, liqueur and sometimes medicines in Europe. The beneficial effects of blackcurrant, which are inhibition of lipopolysaccharide-stimulated inflammatory, anticarcinogenesis and other health effects, have been reported.</p> <p> <italic>Objective</italic>: Previously, we reported the antimutagenic activities of blackcurrant using a yeast gene mutation assay. In this study, we investigated whether this antimutagenicity of blackcurrant was confirmed in human cells.</p> <p> <italic>Materials and methods</italic>: We prepared four types of aqueous blackcurrant extracts (BCE) from mature and premature with or without heat treatment by microwave. Antioxidant activities of BCE were measured by the DPPH radical scavenger assay. In the DPPH radical scavenger assay, the maximum concentration of BCE was 1.6 mg/reaction. We investigated the antigenotoxic activities of BCE by the comet assay and micronucleus test using the human lymphoblastoid cell line TK6. In the comet assay, TK6 was treated with 300 μM H<sub>2</sub>O<sub>2</sub> without or with BCE at concentrations of 0.5, 1.0, 2.0 and 3.0 mg/mL. In the micronucleus test, TK6 was treated with 1 mg/mL BCE without or with H<sub>2</sub>O<sub>2</sub>.</p> <p> <italic>Results</italic>: All BCEs exhibited more than 90% of inhibition rates of DPPH radicals at the maximum concentration of BCE. DNA damage and micronuclei induced by H<sub>2</sub>O<sub>2</sub> significantly decreased in the each BCE-treated condition.</p> <p> <italic>Conclusion</italic>: The results suggest that BCE treatment can reduce the genomic instability induced by H<sub>2</sub>O<sub>2</sub> in human cells. We consider that these antigenotoxic effects are related to polyphenols, <sc>l</sc>-ascorbic acid and other antioxidant compounds.</p> </abstract>