Biodistribution of P-selectin targeted microbubbles. (June 2014)

• Record Type: Journal Article
• Authors: Warram, Jason M.; Sorace, Anna G.; Mahoney, Marshall; Samuel, Sharon; Harbin, Bryant; Joshi, Madhura; Martin, Amber; Whitworth, Lee; Hoyt, Kenneth; Zinn, Kurt R.
• Is Part Of: Journal of drug targeting. Volume 22:Number 5(2014)
• Page Start: 387
• Page End: 394
• Publication Date: 2014-06
• Subjects: Drug delivery systems -- Periodicals; Drug Delivery Systems; Vehicles; Drug Administration Routes; Drug Evaluation; 615.7
• DOI: 10.3109/1061186X.2013.869822
• Languages: English
• ISSNs: 1061-186X

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To evaluate binding of P-selectin targeted microbubbles (MB) in tumor vasculature; a whole-body imaging and biodistribution study was performed in a tumor bearing mouse model.</p> <p> <italic>Methods</italic>: Antibodies were radiolabeled with Tc-99 m using the HYNIC method. Tc-99 m labeled anti-P-selectin antibodies were avidin-bound to lipid-shelled, perfluorocarbon gas-filled MB and intravenously injected into mice bearing MDA-MB-231 breast tumors. Whole-body biodistribution was performed at 5 min (<italic>n</italic> = 12) and 60 min (<italic>n</italic> = 4) using a gamma counter. Tc-99 m-labeled IgG bound IgG-control-MB group (<italic>n</italic> = 12 at 5 min; <italic>n</italic> = 4 at 60 min), Tc-99 m-labeled IgG-control-Ab group (<italic>n</italic> = 5 at 5 min; <italic>n</italic> = 3 at 60 min) and Tc-99 m-labeled anti P-selectin-Ab group (<italic>n</italic> = 5 at 5 min; <italic>n</italic> = 3 at 60 min) were also evaluated. Planar gamma camera imaging was also performed at each time point.</p> <p> <italic>Results</italic>: Targeted-MB retention in tumor (60 min: 1.8 ± 0.3% ID/g) was significantly greater (<italic>p</italic> = 0.01) than targeted-MB levels in adjacent skeletal muscle at both time points (5 min: 0.7 ± 0.2% ID/g; 60 min: 0.2 ± 0.1% ID/g) while there was no significant difference (<italic>p</italic> = 0.17) between muscle and tumor retention for the IgG-control-MB group at 5 min.</p> <p> <italic>Conclusions</italic>: P-selectin targeted MBs were significantly higher in tumor tissue, as compared with adjacent skeletal tissue or tumor retention of IgG-control-MB.</p> </abstract>