Peripheral inflammatory activation after hippocampus irradiation in the rat. (January 2014)

• Record Type: Journal Article
• Authors: Tőkés, Tünde; Varga, Gabriella; Garab, Dénes; Nagy, Zoltán; Fekete, Gábor; Tuboly, Eszter; Plangár, Imola; Mán, Imola; Szabó, Rita Emília; Szabó, Zoltán; Volford, Gábor; Ghyczy, Miklós; Kaszaki, József; Boros, Mihály; Hideghéty, Katalin
• Is Part Of: International journal of radiation biology. Volume 90:Number 1(2014:Jan.)
• Page Start: 1
• Page End: 6
• Publication Date: 2014-01
• Subjects: Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2013.836617
• Languages: English
• ISSNs: 0955-3002

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To detect the possible biochemical signs of inflammatory activation in the peripheral circulation in a rodent model of hippocampus irradiation, and to examine the effects of L-alpha-glycerylphosphorylcholine (GPC) in this experimental protocol.</p> <p> <italic>Materials and methods</italic>: Anesthetized Sprague-Dawley rats were subjected to 40 Gy cobalt irradiation of both hemispheres of the hippocampus, with or without GPC treatment (50 mg/kg intravenously (i.v.), 5 min before the irradiation, <italic>n</italic> = 6, each). A third group (<italic>n</italic> = 6) served as saline-treated control. Blood samples were obtained 3 h after the end of irradiation in order to examine the changes in plasma histamine, tumor necrosis factor-alpha (TNF-α), interleukin 1-beta, interleukin 6 (IL-6) and interleukin 10 (IL-10); liver tissue samples were taken to determine adenosine triphosphate (ATP) concentrations.</p> <p> <italic>Results</italic>: The hepatic ATP levels were significantly declined, while plasma concentrations of circulating TNF-α, IL-6, IL-10 and histamine were significantly increased after hippocampus irradiation. GPC treatment significantly reduced the irradiation-induced release of cytokines and histamine, and the liver ATP level was maintained at the control value.</p> <p> <italic>Conclusions</italic>: Targeted brain irradiation produced measurable pro- and anti-inflammatory cytokine changes in the systemic circulation. GPC supplementation provides significant protection against irradiation-induced peripheral pro-inflammatory activation and ATP depletion.</p> </abstract>