Silver nanoparticles enhance Pseudomonas aeruginosa PAO1 biofilm detachment. (June 2014)

• Record Type: Journal Article
• Authors: Loo, Ching-Yee; Young, Paul M.; Cavaliere, Rosalia; Whitchurch, Cynthia B.; Lee, Wing-Hin; Rohanizadeh, Ramin
• Is Part Of: Drug development and industrial pharmacy. Volume 40:Number 6(2014:Jun.)
• Page Start: 719
• Page End: 729
• Publication Date: 2014-06
• Subjects: Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.3109/03639045.2013.780182
• Languages: English
• ISSNs: 0363-9045

Abstract

<abstract> <title>Abstract</title> <p> <italic>Objectives</italic>: Silver nanoparticles (AgNPs) with a size ranging from 7 to 70 nm were synthesized using the ascorbic acid-citrate seed-mediated growth approach at room temperature.</p> <p> <italic>Methods</italic>: The 8 nm silver particles were prepared using gallic acid in alkaline conditions and used as seed to prepare AgNPs.</p> <p> <italic>Results</italic>: The presence of ascorbic acid and citrate allows the regulation of size and size distribution of the nanoparticles. The increase in free silver ion-to-seed ratio (Ag⁺/Ag⁰) resulted in changes of particle shape from spherical to pseudo-spherical and minor cylindrical shape. Further, a repetitive seeding approach resulted in the formation of pseudo-spherical particles with higher polydispersity index and minor distributions of tetrahedral particles. Citrate-capped AgNPs were stable and did not agglomerate upon centrifugation. The effect of AgNPs on biofilm reduction was evaluated using static culture on 96-well microtiter plates. Results showed that AgNPs with the smallest average diameter were most effective in the reduction of <italic>Pseudomonas aeruginosa</italic> biofilm colonies, which accounted for 90% of removal.</p> <p> <italic>Conclusion</italic>: The biofilm removal activities of the nanoparticles were found to be concentration-independent particularly for the concentration within the range of 80–200 µg/mL.</p> </abstract>