Paired analysis of plasma proteins and coagulant capacity after treatment with three methods of pathogen reduction. Issue 5 (22nd October 2013)

• Record Type: Journal Article
• Authors: Coene, José; Devreese, Katrien; Sabot, Bea; Feys, Hendrik B.; Vandekerckhove, Philippe; Compernolle, Veerle
• Is Part Of: Transfusion. Volume 54:Issue 5(2014)
• Page Start: 1321
• Page End: 1331
• Publication Date: 2013-10-22
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.12460
• Languages: English
• ISSNs: 0041-1132

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="trf12460-sec-0001" sec-type="section"> <title>Background</title> <p>The effect of photochemical pathogen reduction (PR) methods on plasma quality has been the subject of several reports but solid comparative data for the different technologies are lacking.</p> </sec> <sec id="trf12460-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>Plasma (n = 24) photoinactivated with methylene blue (MB), riboflavin (RF), or amotosalen (AS) was compared using a pool‐and‐split design. Samples were taken before and after treatment with each method and tested for coagulation factors (fibrinogen, Factor [F] II, FV, FVIII, F IX, FXI), natural coagulation inhibitors (Protein C [PC], protein S [PS], antithrombin III [AT]), prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin generation (TG). The three methods were mutually compared by repeated‐measures analysis of variance.</p> </sec> <sec id="trf12460-sec-0003" sec-type="section"> <title>Results</title> <p>All three PR methods cause significant reduction (p < 0.01) of activity of the procoagulant proteins fibrinogen, FII, FV, FVIII, F IX, and FXI. Coagulation is also affected, with significant changes in PT, APTT, and TG. RF treatment causes a significantly higher decrease in concentration of coagulation factors, PS, and AT than the other methods (p < 0.01). PT, APTT, and TG are also affected most by RF treatment. FII, FVIII, F IX, PC, AT, and PT are best preserved with the MB method and FV, FXI, and TG after AS treatment (p < 0.01). Coagulation factor loss due to the volume loss during PR treatment is more important for MB and AS than for RF.</p> </sec> <sec id="trf12460-sec-0004" sec-type="section"> <title>Conclusion</title> <p>PR treatment of plasma affects coagulation proteins and coagulant capacity. For the RF method this effect is most pronounced, although to some extent compensated by a smaller volume loss.</p> </sec> </abstract>