Enhanced water and cryoprotectant permeability of porcine oocytes after artificial expression of human and zebrafish aquaporin‐3 channels. Issue 5 (12th March 2014)

• Record Type: Journal Article
• Authors: Morató, Roser; Chauvigné, François; Novo, Sergi; Bonet, Sergi; Cerdà, Joan
• Is Part Of: Molecular reproduction and development. Volume 81:Issue 5(2014:May)
• Page Start: 450
• Page End: 461
• Publication Date: 2014-03-12
• Subjects: Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.22310
• Languages: English
• ISSNs: 1040-452X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>SUMMARY</title> <sec id="mrd22310-sec-0001" sec-type="section"> <p>One of the major obstacles for the vitrification of mature porcine oocytes with ethylene glycol is their low permeability to this cryoprotectant, which results in osmotic stress‐induced cell damage and low survival. Pig blastocysts, on the other hand, show enhanced water and cryoprotectant permeability, which has been related to the transcriptional activation of aquaporin‐3 (AQP3) channels at this stage of development. In this study, we asked if expression of cRNAs encoding two aquaglyceroporins, human AQP3 (hAQP3) or the zebrafish Aqp3b‐T85A mutant, in porcine oocytes can increase their permeability. Microinjection of germinal‐vesicle‐stage oocytes with enhanced green fluorescent protein (<italic>EGFP</italic>) or <italic>AQP3</italic> cRNAs resulted in the expression of the corresponding proteins in ∼26% of the metaphase‐II stage oocytes at 40–44 hr of in vitro culture; co‐injection of <italic>EGFP</italic> cRNA appeared to be a suitable marker for oocyte selection since all EGFP‐positive oocytes also expressed the corresponding aquaporin. Using this method, we found that mature oocytes co‐expressing EGFP and hAQP3 or EGFP and Aqp3b‐T85A showed approximately a twofold increase of the hydraulic conductivity (L<sub>p</sub>) with respect non‐injected or <italic>EGFP</italic> alone‐injected oocytes in a 0.43 M sucrose or 1.3 M ethylene glycol solution, whereas the ethylene glycol permeability (P<sub>EG</sub>) of EGFP + hAQP3 and EGFP + Aqp3b‐T85A oocytes was 6.7‐ and 12‐fold higher, respectively, than control oocytes. These data demonstrate that the artificial expression of aquaglyceroporins in porcine metaphase‐II oocytes improves their permeability, and that the zebrafish Aqp3b‐T85A mutant is more efficient than the human channel at increasing the oocyte permeability to ethylene glycol. <italic>Mol. Reprod. Dev</italic>. 81: ???–???, 2014. © 2014 Wiley Periodicals, Inc.</p> </sec> </abstract>