Assessing intrarenal nonperfusion and vascular leakage in acute kidney injury with multinuclear 1H/19F MRI and perfluorocarbon nanoparticles. Issue 6 (8th August 2013)

• Record Type: Journal Article
• Authors: Hu, Lingzhi; Chen, Junjie; Yang, Xiaoxia; Senpan, Angana; Allen, John S.; Yanaba, Noriko; Caruthers, Shelton D.; Lanza, Gregory M.; Hammerman, Marc R.; Wickline, Samuel A.
• Is Part Of: Magnetic resonance in medicine. Volume 71:Issue 6(2014:Jun.)
• Page Start: 2186
• Page End: 2196
• Publication Date: 2013-08-08
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.24851
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm24851-sec-0001" sec-type="section"> <title>Purpose</title> <p>We sought to develop a unique sensor‐reporter approach for functional kidney imaging that employs circulating perfluorocarbon nanoparticles and multinuclear <sup>1</sup>H/<sup>19</sup>F MRI.</p> </sec> <sec id="mrm24851-sec-0002" sec-type="section"> <title>Methods</title> <p> <sup>19</sup>F spin density weighted and T1 weighted images were used to generate quantitative functional mappings of both healthy and ischemia‐reperfusion (acute kidney injury) injured mouse kidneys. <sup>1</sup>H blood‐oxygenation‐level‐dependent (BOLD) MRI was also employed as a supplementary approach to facilitate the comprehensive analysis of renal circulation and its pathological changes in acute kidney injury.</p> </sec> <sec id="mrm24851-sec-0003" sec-type="section"> <title>Results</title> <p>Heterogeneous blood volume distributions and intrarenal oxygenation gradients were confirmed in healthy kidneys by <sup>19</sup>F MRI. In a mouse model of acute kidney injury, <sup>19</sup>F MRI, in conjunction with blood‐oxygenation‐level‐dependent MRI, sensitively delineated renal vascular damage and recovery. In the cortico‐medullary junction region, we observed 25% lower <sup>19</sup>F signal (<italic>P</italic> < 0.05) and 70% longer <sup>1</sup>H T2* (<italic>P</italic> < 0.01) in injured kidneys compared with contralateral kidneys at 24 h after initial ischemia‐reperfusion injury. We also detected 71% higher <sup>19</sup>F signal (<italic>P</italic> < 0.01) and 40% lower <sup>1</sup>H T2* (<italic>P</italic> < 0.05) in the renal medulla region of injured kidneys compared with contralateral uninjured kidneys.</p> </sec> <sec id="mrm24851-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Integrated <sup>1</sup>H/<sup>19</sup>F MRI using perfluorocarbon nanoparticles provides a multiparametric readout of regional perfusion defects in acutely injured kidneys. <bold>Magn Reson Med 71:2186–2196, 2014. © 2013 Wiley Periodicals, Inc.</bold></p> </sec> </abstract>