Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases. Issue 5 (19th March 2014)

• Record Type: Journal Article
• Authors: Yoshida, Keita; Treen, Nicholas; Hozumi, Akiko; Sakuma, Tetsushi; Yamamoto, Takashi; Sasakura, Yasunori
• Is Part Of: Genesis. Volume 52:Issue 5(2014:May)
• Page Start: 431
• Page End: 439
• Publication Date: 2014-03-19
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22770
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Summary: Targeted mutagenesis of genes‐of‐interest, or gene‐knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian <italic>Ciona intestinalis</italic> is an excellent organism to analyze gene functions by means of genetic technologies. In our previous study, we reported mutagenesis of <italic>Ciona</italic> somatic cells with TALE nucleases (TALENs) by electroporating expression constructs. In this study, we report germ cell mutagenesis of <italic>Ciona</italic> by microinjecting mRNAs encoding TALENs. TALEN mRNAs introduced mutations to target genes in both somatic and germ cells. TALEN‐mediated mutations in the germ cell genome were inherited by the next generation. We conclude that knockout lines of <italic>Ciona</italic> that have disrupted target genes can be established through TALEN‐mediated germ cell mutagenesis. genesis 52:431–439, 2014. © 2014 Wiley Periodicals, Inc.</p> </abstract>